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PMID: 1945859 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

DMSO-enhanced whole cell yeast transformation.

Nucleic acids research ·Vol. 19 ·No. 20 ·1991-10-25 ·Pages 5791

Hill J, Donald KA, Griffiths DE, Donald G

Abstract

暂无摘要

MeSH Terms

Dimethyl Sulfoxide/pharmacology Saccharomyces cerevisiae/drug effects,genetics Transformation, Genetic/drug effects

Chemicals

Dimethyl Sulfoxide

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Hill J

Department of Chemistry, University of Warwick, Coventry, UK.

Donald K A

Griffiths D E

Donald G

References (9)

9 references, click to expand

Transformation of intact yeast cells treated with alkali cations.
J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment.
Nucleic Acids Res. 1984 Jul 25;12(14):5707-17 PMID: 6589587
A rapid and convenient method for the preparation and storage of competent bacterial cells.
Nucleic Acids Res. 1988 Apr 25;16(8):3580 PMID: 3287331
Specificity of DNA uptake during whole cell transformation of S. cerevisiae.
Yeast. 1987 Jun;3(2):131-7 PMID: 3332965
Transformation of yeast by a replicating hybrid plasmid.
Nature. 1978 Sep 14;275(5676):104-9 PMID: 357984
High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier.
Curr Genet. 1989 Dec;16(5-6):339-46 PMID: 2692852
G418-resistance as a dominant marker and reporter for gene expression in Saccharomyces cerevisiae.
Curr Genet. 1990 Nov;18(4):303-13 PMID: 2174744
High-efficiency transformation of yeast by electroporation.
Methods Enzymol. 1991;194:182-7 PMID: 2005786
Yeast/E. coli shuttle vectors with multiple unique restriction sites.
Yeast. 1986 Sep;2(3):163-7 PMID: 3333305

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/19.20.5791

Published

1991-10-25

Pages

5791

Language

English

Region

England

NLM ID

0411011

PMCID

PMC328997

Subset

Corrections

ErratumIn

External Links

PubMed source DOI: 10.1093/nar/19.20.5791 PMC Full Text View journal details

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