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PMID: 19451398 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

IL-18 and IL-33 elicit Th2 cytokines from basophils via a MyD88- and p38alpha-dependent pathway.

Journal of leukocyte biology ·Vol. 86 ·No. 4 ·2009-10-00 ·Pages 769-78

Kroeger KM, Sullivan BM, Locksley RM

Abstract

IL-4 and IL-13 are instrumental in the development and progression of allergy and atopic disease. Basophils represent a key source of these cytokines and produce IL-4 and IL-13 when stimulated with IL-18, a member of the IL-1 family of cytokines. Comparative analyses of the effects of caspase-1-dependent IL-1 family cytokines on basophil IL-4 and IL-13 production have not been performed, and the signaling pathway proteins required for FcepsilonRI-independent Th2 cytokine production from basophils remain incompletely defined. Using mouse bone marrow-derived cultured basophils, we found that IL-4 and IL-13 are produced in response to IL-18 or IL-33 stimulation. IL-18- or IL-33-mediated Th2 cytokine production is dependent on MyD88 and p38alpha signaling proteins. In addition, basophil survival increased in the presence of IL-18 or IL-33 as a result of increased Akt activation. Studies in vivo confirmed the potency of IL-18 and IL-33 in activating cytokine release from mouse basophils.

MeSH Terms
Animals Basophils/cytology,immunology Cell Survival/drug effects,immunology Cells, Cultured Female Interleukin-13/immunology Interleukin-18/immunology,pharmacology Interleukin-33 Interleukin-4/immunology Interleukins/immunology,pharmacology Mice Mice, Inbred BALB C Mice, Knockout Mitogen-Activated Protein Kinase 14/immunology Myeloid Differentiation Factor 88/immunology Proto-Oncogene Proteins c-akt/immunology Signal Transduction/drug effects,immunology Th2 Cells/immunology
Chemicals
Il33 protein, mouse Interleukin-13 Interleukin-18 Interleukin-33 Interleukins Myd88 protein, mouse Myeloid Differentiation Factor 88 Interleukin-4 Proto-Oncogene Proteins c-akt Mitogen-Activated Protein Kinase 14
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kroeger Kelly M
Howard Hughes Medical Institute, Departments of Medicine and Microbiology/Immunology, University of California San Francisco, San Francisco, California, USA.
Sullivan Brandon M
Locksley Richard M
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Article Info
Journal
Journal of leukocyte biology
Abbr.
J Leukoc Biol
ISSN
1938-3673
DOI
10.1189/jlb.0708452
Published
2009-10-00
Epub
2009-00-18
Pages
769-78
Language
English
Region
United States
NLM ID
8405628
PMCID
PMC2752018
Subset
IM
Grants
NIAID NIH HHS · R01 AI026918 · United States
NIAID NIH HHS · R37 AI026918 · United States
Howard Hughes Medical Institute · United States
NIAID NIH HHS · AI026918 · United States
Corrections
CommentIn
PMID: 19797302
External Links
PubMed source DOI: 10.1189/jlb.0708452 PMC Full Text View journal details
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