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PMID: 1944227 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The CCR1 (SNF1) and SCH9 protein kinases act independently of cAMP-dependent protein kinase and the transcriptional activator ADR1 in controlling yeast ADH2 expression.

Molecular & general genetics : MGG ·Vol. 229 ·No. 3 ·1991-10-00 ·Pages 395-9

Denis CL, Audino DC

Abstract

cAMP-dependent protein kinase (cAPK) is implicated in the inactivation of the yeast transcriptional activator ADR1, which regulates glucose-repressible ADH2 gene expression. The interdependence of cAPK, SCH9 (a protein kinase that when overexpressed can functionally substitute for cAPK), and the CCR1 (SNF1) protein kinase that is required for ADH2 expression was studied. SCH9 was found to be required for ADH2 expression in contrast to the inhibitory role played by cAPK. CCR1 and SCH9 were observed to affect ADH2 expression independently of both ADR1 and cAPK. In contrast, cAPK was shown to exert its effects on ADH2 solely through ADR1. These results indicate that the SCH9 and CCR1 protein kinases are components of regulatory pathways separate from that utilized by cAPK to control ADR1 activity and ADH2 expression.

MeSH Terms
Alcohol Dehydrogenase/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Fungal Proteins/metabolism Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Protein Kinases/metabolism Protein Serine-Threonine Kinases Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins Transcription Factors/metabolism
Chemicals
ADR1 protein, S cerevisiae DNA-Binding Proteins Fungal Proteins Saccharomyces cerevisiae Proteins Transcription Factors Alcohol Dehydrogenase Protein Kinases SCH9 protein kinase SNF1-related protein kinases Protein Serine-Threonine Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Denis C L
Department of Biochemistry, University of New Hampshire, Durham 03824.
Audino D C
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267461
Published
1991-10-00
Pages
395-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM41215 · United States
External Links
PubMed source DOI: 10.1007/BF00267461
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