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PMID: 1942242 Published · ppublish English Journal Article

Transactivation of human immunodeficiency virus type 1 long terminal repeats by cell surface tumor necrosis factor alpha.

Journal of virology ·Vol. 65 ·No. 12 ·1991-12-00 ·Pages 6425-9

Tadmori W, Mondal D, Tadmori I, Prakash O

Abstract

Tumor necrosis factor alpha (TNF-alpha) is expressed in secreted and cell surface (csTNF-alpha) forms by activated monocytic and T cells. In this report, we demonstrate that csTNF-alpha may predominantly regulate the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) activation in the promonocytic cell line U937 and in the Epstein-Barr virus-transformed B-cell line BH1. Anti-TNF-alpha antibody suppressed both the constitutive expression of the HIV-1 LTR in BH1 cells and the expression induced by phorbol 12-myristate 13-acetate in U937 cells. This suppression was found to be mediated via csTNF-alpha. No correlation between the HIV-1 LTR activation and the secretion of TNF-alpha was evident in these cell lines. Suppression of TNF-alpha secretion by cyclosporin A or by a serine protease inhibitor did not suppress the HIV-1 LTR activation. These observations suggest a novel biological role for csTNF-alpha in the immunopathogenesis of AIDS.

MeSH Terms
Cell Line Flow Cytometry HIV Long Terminal Repeat HIV-1/drug effects,genetics Humans Kinetics Tetradecanoylphorbol Acetate/pharmacology Transcriptional Activation/drug effects Transfection Tumor Necrosis Factor-alpha/genetics,pharmacology,physiology
Chemicals
Tumor Necrosis Factor-alpha Tetradecanoylphorbol Acetate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tadmori W
Department of Molecular Oncology, Alton Ochsner Medical Foundation, New Orleans, Louisiana 70121.
Mondal D
Tadmori I
Prakash O
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.12.6425-6429.1991
Published
1991-12-00
Pages
6425-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC250679
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.65.12.6425-6429.1991 PMC Full Text View journal details
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