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PMID: 19420357 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Surface antigen phenotypes of hematopoietic stem cells from embryos and murine embryonic stem cells.

Blood ·Vol. 114 ·No. 2 ·2009-07-09 ·Pages 268-78

McKinney-Freeman SL, Naveiras O, Yates F, Loewer S, Philitas M, Curran M, Park PJ, Daley GQ

Abstract

Surface antigens on hematopoietic stem cells (HSCs) enable prospective isolation and characterization. Here, we compare the cell-surface phenotype of hematopoietic repopulating cells from murine yolk sac, aorta-gonad-mesonephros, placenta, fetal liver, and bone marrow with that of HSCs derived from the in vitro differentiation of murine embryonic stem cells (ESC-HSCs). Whereas c-Kit marks all HSC populations, CD41, CD45, CD34, and CD150 were developmentally regulated: the earliest embryonic HSCs express CD41 and CD34 and lack CD45 and CD150, whereas more mature HSCs lack CD41 and CD34 and express CD45 and CD150. ESC-HSCs express CD41 and CD150, lack CD34, and are heterogeneous for CD45. Finally, although CD48 was absent from all in vivo HSCs examined, ESC-HSCs were heterogeneous for the expression of this molecule. This unique phenotype signifies a developmentally immature population of cells with features of both primitive and mature HSC. The prospective fractionation of ESC-HSCs will facilitate studies of HSC maturation essential for normal functional engraftment in irradiated adults.

MeSH Terms
Animals Antigens, CD/analysis,metabolism Cells, Cultured Embryo, Mammalian/metabolism Embryonic Stem Cells/metabolism Female Hematopoietic Stem Cells/metabolism Mice Mice, Inbred C57BL Phenotype Placenta/metabolism
Chemicals
Antigens, CD
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
McKinney-Freeman Shannon L
Division of Pediatric Hematology/Oncology, Manton Center for Orphan Diseases, Stem Cell Transplantation Program, Children's Hospital, Boston, MA 02115, USA.
Naveiras Olaia
Yates Frank
Loewer Sabine
Philitas Marsha
Curran Matthew
Park Peter J
Daley George Q
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Article Info
Journal
Blood
Abbr.
Blood
ISSN
1528-0020
DOI
10.1182/blood-2008-12-193888
Published
2009-07-09
Epub
2009-00-06
Pages
268-78
Language
English
Region
United States
NLM ID
7603509
PMCID
PMC2714203
Subset
IM
External Links
PubMed source DOI: 10.1182/blood-2008-12-193888 PMC Full Text View journal details
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