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PMID: 19419240 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure.

PLoS biology ·Vol. 7 ·No. 5 ·2009-05-00 ·Pages e1000102

Korkhin Y, Unligil UM, Littlefield O, Nelson PJ, Stuart DI, Sigler PB, Bell SD, Abrescia NG

Abstract

The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfolobus shibatae at 3.35 Å resolution. The structure contains the full complement of subunits, including RpoG/Rpb8 and the equivalent of the clamp-head and jaw domains of the eukaryotic Rpb1. Furthermore, we have identified subunit Rpo13, an RNAP component in the order Sulfolobales, which contains a helix-turn-helix motif that interacts with the RpoH/Rpb5 and RpoA'/Rpb1 subunits. Its location and topology suggest a role in the formation of the transcription bubble.

MeSH Terms
Amino Acid Sequence Archaeal Proteins/chemistry,genetics,metabolism Crystallography, X-Ray DNA-Directed RNA Polymerases/chemistry,genetics,metabolism Evolution, Molecular Models, Molecular Molecular Sequence Data Protein Subunits/chemistry,genetics,metabolism RNA, Archaeal/genetics,metabolism Sulfolobus/chemistry,enzymology,genetics Transcription, Genetic
Chemicals
Archaeal Proteins Protein Subunits RNA, Archaeal DNA-Directed RNA Polymerases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Korkhin Yakov
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, USA.
Unligil Ulug M
Littlefield Otis
Nelson Pamlea J
Stuart David I
Sigler Paul B
Bell Stephen D
Abrescia Nicola G A
Conflict of Interest

Competing interests. The authors have declared that no competing interests exist.

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Article Info
Journal
PLoS biology
Abbr.
PLoS Biol
ISSN
1545-7885
DOI
10.1371/journal.pbio.1000102
Published
2009-05-00
Epub
2009-00-05
Pages
e1000102
Language
English
Region
United States
NLM ID
101183755
PMCID
PMC2675907
Subset
IM
Grants
Medical Research Council · G0500365 · United Kingdom
Howard Hughes Medical Institute · United States
External Links
PubMed source DOI: 10.1371/journal.pbio.1000102 PMC Full Text View journal details
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