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PMID: 19414609 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Role of the Ndc1 interaction network in yeast nuclear pore complex assembly and maintenance.

The Journal of cell biology ·Vol. 185 ·No. 3 ·2009-05-04 ·Pages 475-91

Onischenko E, Stanton LH, Madrid AS, Kieselbach T, Weis K

Abstract

The nuclear pore complex (NPC) mediates all nucleocytoplasmic transport, yet its structure and biogenesis remain poorly understood. In this study, we have functionally characterized interaction partners of the yeast transmembrane nucleoporin Ndc1. Ndc1 forms a distinct complex with the transmembrane proteins Pom152 and Pom34 and two alternative complexes with the soluble nucleoporins Nup53 and Nup59, which in turn bind to Nup170 and Nup157. The transmembrane and soluble Ndc1-binding partners have redundant functions at the NPC, and disruption of both groups of interactions causes defects in Ndc1 targeting and in NPC structure accompanied by significant pore dilation. Using photoconvertible fluorescent protein fusions, we further show that the depletion of Pom34 in cells that lack NUP53 and NUP59 blocks new NPC assembly and leads to the reversible accumulation of newly made nucleoporins in cytoplasmic foci. Therefore, Ndc1 together with its interaction partners are collectively essential for the biosynthesis and structural integrity of yeast NPCs.

MeSH Terms
Cell Survival Cytoplasm/physiology Kinetics Luminescent Proteins/genetics Membrane Glycoproteins/metabolism Methionine/metabolism Nuclear Pore/physiology,ultrastructure Nuclear Pore Complex Proteins/deficiency,genetics,metabolism Nuclear Proteins/deficiency,genetics,metabolism,physiology Recombinant Fusion Proteins/metabolism Saccharomyces cerevisiae/cytology,physiology,ultrastructure Saccharomyces cerevisiae Proteins/genetics,metabolism,physiology
Chemicals
Luminescent Proteins Membrane Glycoproteins NDC1 protein, S cerevisiae NUP53 protein, S cerevisiae Nuclear Pore Complex Proteins Nuclear Proteins POM152 protein, S cerevisiae Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Methionine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Onischenko Evgeny
Department of Molecular and Cell Biology, Division of Cell and Developmental Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Stanton Leslie H
Madrid Alexis S
Kieselbach Thomas
Weis Karsten
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
1540-8140
DOI
10.1083/jcb.200810030
Published
2009-05-04
Pages
475-91
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2700399
Subset
IM
Grants
NIGMS NIH HHS · R01 GM058065 · United States
NIGMS NIH HHS · GM58065 · United States
Corrections
CommentIn
PMID: 19414605
External Links
PubMed source DOI: 10.1083/jcb.200810030 PMC Full Text View journal details
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