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PMID: 1937799 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Helminthotoxic responses of intestinal eosinophils to Trichinella spiralis newborn larvae.

Infection and immunity ·Vol. 59 ·No. 12 ·1991-12-00 ·Pages 4405-11

Lee TD

Abstract

Because the gastrointestinal lamina propria is the first line of defense against invasion with Trichinella spiralis newborn larvae, we investigated the helminthotoxic characteristics of isolated lamina propria eosinophils. Eosinophils were isolated from the intestinal lamina propria of rats and purified to nearly 90% purity by a combination of velocity sedimentation through Percoll and unit gravity sedimentation through a continuous gradient of bovine serum albumin. Isolated eosinophils were of high viability and responded to surface receptor stimulation. Freshly isolated intestinal eosinophils lacked cytotoxic capacity when incubated with newborn larvae in the presence of specific antiserum. Peritoneal eosinophils from the same rats exhibited 100% helminthotoxicity after 24 h. Cytotoxicity could be stimulated in the intestinal eosinophils by the addition of recombinant murine interleukin-5.

MeSH Terms
Animals Cell Adhesion Cell Separation Cytotoxicity, Immunologic Eosinophils/immunology Glucuronidase/metabolism Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology In Vitro Techniques Interleukin-5/pharmacology Intestines/immunology,parasitology Larva/immunology Male Rats Rats, Inbred Strains Trichinella/immunology
Chemicals
Interleukin-5 Granulocyte-Macrophage Colony-Stimulating Factor Glucuronidase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Lee T D
Department of Microbiology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada.
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24 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.59.12.4405-4411.1991
Published
1991-12-00
Pages
4405-11
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259056
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.59.12.4405-4411.1991 PMC Full Text View journal details
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