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PMID: 19374999 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Chapter 23. Cyanobactin ribosomally synthesized peptides--a case of deep metagenome mining.

Methods in enzymology ·Vol. 458 ·2009-00-00 ·Pages 575-96

Schmidt EW, Donia MS

Abstract

Deep metagenome mining is a new method for engineering natural product pathways, focusing on examining symbiotic organisms. The method has been applied to a family of compounds known as cyanobactins, which are ribosomally synthesized peptides produced by cyanobacteria. Often, these cyanobacteria live symbiotically with marine animals, leading to production of natural products in whole animal samples. Here, we focus on methods to identify, clone, and study cyanobactin natural product genes from axenic organisms and metagenomic environments. The application to deep metagenome mining is described, along with other potential targets of this methodology.

MeSH Terms
Cyanobacteria/genetics,metabolism Genome, Bacterial/genetics,physiology Peptide Biosynthesis/genetics Peptides/genetics,metabolism Prochloron/genetics,metabolism Ribosomes/genetics,metabolism
Chemicals
Peptides
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schmidt Eric W
Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah, USA.
Donia Mohamed S
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Article Info
Journal
Methods in enzymology
Abbr.
Methods Enzymol
ISSN
1557-7988
DOI
10.1016/S0076-6879(09)04823-X
Published
2009-00-00
Pages
575-96
Language
English
Region
United States
NLM ID
0212271
PMCID
PMC2810138
Subset
IM
Grants
NIGMS NIH HHS · GM071425 · United States
NIGMS NIH HHS · R01 GM071425 · United States
NIGMS NIH HHS · R01 GM071425-01A1 · United States
NIGMS NIH HHS · R01 GM071425-04 · United States
NIGMS NIH HHS · R01 GM071425-03 · United States
NIGMS NIH HHS · R01 GM071425-02 · United States
External Links
PubMed source DOI: 10.1016/S0076-6879(09)04823-X PMC Full Text
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