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PMID: 19370066 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification and potential enzyme capacity of flavobacteria isolated from the rhizosphere of barley (Hordeum vulgare L.).

Canadian journal of microbiology ·Vol. 55 ·No. 3 ·2009-03-00 ·Pages 234-41

Johansen JE, Nielsen P, Binnerup SJ

Abstract

The diversity of 99 flavobacterium-like isolates from a barley rhizosphere is described. They were identified on 1/10 strength tryptic soy agar by their yellowish colour and a flexirubin reaction after exposure to 10% KOH.16S rDNA partial sequencing identified the majority (70%) of isolates as Flavobacterium. Twelve percent of the isolates belonged to other genera in the phylum Bacteroidetes. Finally 17% of the isolates did not belong to the phylum Bacteroidetes. Most of the Flavobacterium isolates were affiliated to various aquatic, validly named species and likely represent a bulk of undescribed soil Flavobacterium species found in especially high numbers in the rhizosphere of young plant roots. Most Flavobacterium isolates showed gliding motility on CY agar and VY/2 agar, whereas none of the other isolates shared this feature. A high percentage of Flavobacterium strains produced enzymes involved in polysaccharide and protein digestion as well as extracellular phosphatases, compared with strains related to other genera in the phylum Bacteroidetes.

MeSH Terms
Bacterial Typing Techniques DNA, Bacterial/analysis DNA, Ribosomal Flavobacteriaceae/classification,enzymology,genetics,isolation & purification Hordeum/microbiology Molecular Sequence Data Movement Phenotype Phylogeny Plant Roots/microbiology RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Soil Microbiology
Chemicals
DNA, Bacterial DNA, Ribosomal RNA, Ribosomal, 16S
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Johansen Jens Efsen
Department of Environmental Chemistry and Microbiology, National Environmental Research Institute, DK-4000 Roskilde, Denmark. JnJo@novonordisk.com
Nielsen Preben
Binnerup Svend Jørgen
Article Info
Journal
Canadian journal of microbiology
Abbr.
Can J Microbiol
ISSN
0008-4166
DOI
10.1139/w08-116
Email
jnjo@novonordisk.com
Published
2009-03-00
Pages
234-41
Language
English
Region
Canada
NLM ID
0372707
Subset
IM
Databases
GENBANK
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External Links
PubMed source DOI: 10.1139/w08-116 View journal details
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