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PMID: 19364820 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Spatially restricted translation of the xCR1 mRNA in Xenopus embryos.

Molecular and cellular biology ·Vol. 29 ·No. 13 ·2009-07-00 ·Pages 3791-802

Zhang Y, Forinash KD, McGivern J, Fritz B, Dorey K, Sheets MD

Abstract

The xCR1 protein is a maternal determinant and cofactor for nodal signaling in vertebrate embryos. The xCR1 protein accumulates specifically in the animal cells of Xenopus embryos, but maternal xCR1 mRNA is distributed equally throughout all embryonic cells. Here, we show that vegetal cell-specific translational repression of xCR1 mRNA contributes to this spatially restricted accumulation of the xCR1 protein in Xenopus embryos. xCR1 mRNA was associated with polyribosomes in animal cells but not vegetal cells. A 351-nucleotide region of xCR1 mRNA's 3' untranslated region was sufficient to confer a spatially restricted pattern of translation to a luciferase reporter mRNA by repressing translation in vegetal cells. Repression depended upon the mRNA's 5' cap but not its 3' poly(A) tail. Furthermore, the region of xCR1 mRNA sufficient to confer vegetal cell-specific repression contained both Pumilio binding elements (PBEs) and binding sites for the CUG-BP1 protein. The PBEs and the CUG-BP1 sites were necessary but not sufficient for translation repression. Our studies of xCR1 mRNA document the first example of spatially regulated translation in controlling the asymmetric distribution of a maternal determinant in vertebrates.

MeSH Terms
3' Untranslated Regions Animals Base Sequence Cell Polarity Gene Expression Regulation, Developmental Genes, Reporter Membrane Proteins/genetics,metabolism Molecular Sequence Data Oocytes/cytology,physiology Poly A/genetics,metabolism Polyribosomes/genetics,metabolism Protein Biosynthesis RNA Caps/metabolism RNA, Messenger/genetics,metabolism RNA-Binding Proteins/genetics,metabolism Xenopus Proteins/genetics,metabolism Xenopus laevis/anatomy & histology,embryology,physiology
Chemicals
3' Untranslated Regions Membrane Proteins PUM1 protein, Xenopus RNA Caps RNA, Messenger RNA-Binding Proteins Xenopus Proteins tdgf1.3 protein, Xenopus Poly A
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Zhang Yan
University of Wisconsin-Madison, Department of Biomolecular Chemistry, 1300 University Avenue, Madison, WI 53706, USA.
Forinash Kara D
McGivern Jered
Fritz Brian
Dorey Karel
Sheets Michael D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
1098-5549
DOI
10.1128/MCB.01865-08
Published
2009-07-00
Epub
2009-00-13
Pages
3791-802
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC2698763
Subset
IM
Grants
NICHD NIH HHS · R01 HD043996 · United States
NICHD NIH HHS · HD43996 · United States
External Links
PubMed source DOI: 10.1128/MCB.01865-08 PMC Full Text View journal details
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