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题目:: Epigenetics of multiple myeloma after treatment with cytostatics and gamma radiation.
作者:: Krejcí(Jana),Harnicarová(Andrea),Streitová(Denisa),Hájek(Roman),Pour(Ludek),Kozubek(Stanislav),Bártová(Eva)
状态:: 发布时间2009-08-21 , 更新时间 2009-08-21
期刊:: Leuk Res
摘要:: Genetic and epigenetic changes in multiple myeloma (MM) correlate with the stage of the disease. Therefore, we investigated how cytostatics and gamma radiation influence MM-associated histone modifications. ChIP-PCR and ChIP-on-chip technologies were used to quantify H3K9 acetylation and H3K9 dimethylation at select loci in MM patients, lymphoblastoid ARH-77, and myeloma MOLP-8 cells. Genome-wide analysis revealed that the cytostatic, melphalan, increased H3K9 acetylation at multiple gene promoters in ARH-77 cells. Melphalan and gamma radiation also influenced histone modification of prognostically important c-myc and CCND1 genes in ARH-77 and MOLP-8 cells. Moreover, H3K9 acetylation at c-myc and CCND1 promoters was increased in individual MM patients after melphalan treatment. Western blotting revealed that these effects were accompanied by changes in c-MYC and cyclin D1 protein levels. Taken together, we showed that cytostatics significantly alter histone modification of tumor-related genes which is indispensable for understanding cancer therapies.
语言:: eng
DOI:: 10.1016/j.leukres.2009.03.016

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