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PMID: 19339499 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A homolog of human ski-interacting protein in rice positively regulates cell viability and stress tolerance.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 106 ·No. 15 ·2009-04-14 ·Pages 6410-5

Hou X, Xie K, Yao J, Qi Z, Xiong L

Abstract

Abiotic stresses are major limiting factors for growth, development, and productivity of crop plants. Here, we report on OsSKIPa, a rice homolog of human Ski-interacting protein (SKIP) that can complement the lethal defect of the knockout mutant of SKIP homolog in yeast and positively modulate cell viability and stress tolerance of rice. Suppression of OsSKIPa in rice resulted in growth arrest and reduced cell viability. The expression OsSKIPa is induced by various abiotic stresses and phytohormone treatments. Transgenic rice overexpressing OsSKIPa exhibited significantly improved growth performance in the medium containing stress agents (abscisic acid, salt, or mannitol) and drought resistance at both the seedling and reproductive stages. The OsSKIPa-overexpressing rice showed significantly increased reactive oxygen species-scavenging ability and transcript levels of many stress-related genes, including SNAC1 and rice homologs of CBF2, PP2C, and RD22, under drought stress conditions. More than 30 OsSKIPa-interacting proteins were identified, but most of these proteins have no matches with the reported SKIP-interacting proteins in animals and yeast. Together, these data suggest that OsSKIPa has evolved a specific function in positive modulation of stress resistance through transcriptional regulation of diverse stress-related genes in rice.

MeSH Terms
Adaptation, Physiological/drug effects Cell Survival Gene Expression Regulation, Plant Humans Molecular Sequence Data Mutation/genetics Nuclear Proteins/genetics,metabolism Oryza/cytology,genetics,growth & development,metabolism Phytochrome/metabolism Plant Proteins/genetics,metabolism Plants, Genetically Modified Reactive Oxygen Species/metabolism Saccharomyces cerevisiae/genetics,metabolism Transcription Factors/genetics,metabolism
Chemicals
Nuclear Proteins Plant Proteins Reactive Oxygen Species Transcription Factors Phytochrome
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hou Xin
National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China.
Xie Kabin
Yao Jialing
Qi Zhuyun
Xiong Lizhong
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.0901940106
Published
2009-04-14
Epub
2009-00-01
Pages
6410-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2669339
Subset
IM
Databases
GENBANK
AK067153, EU368691, EU368692, EU368693, EU368694, EU368695, EU368696, EU368697, EU368698, EU368699, EU368700, EU368701, EU368702, EU368703, EU368704, EU368705, EU368706, EU368707, EU368708, EU368709, EU368710, EU368711, EU368712, EU368713, EU368714, EU368715, EU368716, EU368717, EU368718, EU368719, EU368720, EU368721, EU368722, EU368723, EU368724, EU368725, GSE10054
External Links
PubMed source DOI: 10.1073/pnas.0901940106 PMC Full Text View journal details
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