Home LiteratureArticle Details
PMID: 1932550 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Measurement of mass transport and reaction parameters in bulk solution using photobleaching. Reaction limited binding regime.

Biophysical journal ·Vol. 60 ·No. 3 ·1991-09-00 ·Pages 596-610

Kaufman EN, Jain RK

Abstract

Fluorescence recovery after photobleaching (FRAP) has been used previously to investigate the kinetics of binding to biological surfaces. The present study adapts and further develops this technique for the quantification of mass transport and reaction parameters in bulk media. The technique's ability to obtain the bulk diffusion coefficient, concentration of binding sites, and equilibrium binding constant for ligand/receptor interactions in the reaction limited binding regime is assessed using the B72.3/TAG-72 monoclonal antibody/tumor associated antigen interaction as a model in vitro system. Measurements were independently verified using fluorometry. The bulk diffusion coefficient, concentration of binding sites and equilibrium binding constant for the system investigated were 6.1 +/- 1.1 x 10(-7) cm2/s, 4.4 +/- 0.6 x 10(-7) M, and 2.5 +/- 1.6 x 10(7) M-1, respectively. Model robustness and the applicability of the technique for in vivo quantification of mass transport and reaction parameters are addressed. With a suitable animal model, it is believed that this technique is capable of quantifying mass transport and reaction parameters in vivo.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antigens, Neoplasm/immunology Binding Sites, Antibody Cell Membrane/immunology,metabolism Immunoglobulin G/metabolism Kinetics Mathematics Mice Models, Biological Photochemistry Solutions Spectrometry, Fluorescence/methods
Chemicals
Antibodies, Monoclonal Antigens, Neoplasm Immunoglobulin G Solutions
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kaufman E N
Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213-3890.
Jain R K
References (51)
51 references, click to expand
  1. Immunoglobulin surface-binding kinetics studied by total internal reflection with fluorescence correlation spectroscopy.
    Biophys J. 1983 Jul;43(1):103-14 PMID: 6882857
  2. An enzyme-linked immunosorbent assay for measuring anti-sheep erythrocyte antibodies.
    J Immunol Methods. 1984 Mar 30;68(1-2):193-204 PMID: 6368693
  3. Determination of the immunoreactive fraction of radiolabeled monoclonal antibodies by linear extrapolation to binding at infinite antigen excess.
    J Immunol Methods. 1984 Aug 3;72(1):77-89 PMID: 6086763
  4. Monoclonal antibodies to carcinoembryonic antigen: a systematic analysis of antibody specificities by using related normal antigens and evidence for allotypic determinants on carcinoembryonic antigen.
    J Immunol. 1984 Oct;133(4):2090-7 PMID: 6206151
  5. Influence of spatial configuration of carcinoma cell populations on the expression of a tumor-associated glycoprotein.
    Cancer Res. 1985 Feb;45(2):833-40 PMID: 3881173
  6. Surface expression of tumor-associated antigens in primary cultured human colonic epithelial cells from carcinomas, benign tumors, and normal tissues.
    Cancer Res. 1985 Nov;45(11 Pt 2):5648-55 PMID: 2413994
  7. Optimization of monoclonal antibody delivery via the lymphatics: the dose dependence.
    Cancer Res. 1986 Apr;46(4 Pt 1):1830-4 PMID: 3948166
  8. Pharmacokinetics of monoclonal immunoglobulin G1, F(ab')2, and Fab' in mice.
    Cancer Res. 1986 Aug;46(8):3969-78 PMID: 3731067
  9. Penetration and binding of radiolabeled anti-carcinoembryonic antigen monoclonal antibodies and their antigen binding fragments in human colon multicellular tumor spheroids.
    Cancer Res. 1987 Mar 15;47(6):1627-33 PMID: 3545451
  10. Kinetic model for the biodistribution of an 111In-labeled monoclonal antibody in humans.
    Cancer Res. 1987 Jun 15;47(12):3328-36 PMID: 3581071
  11. Method of analysis of non-competitive enzyme immunoassays for antibody quantification.
    J Immunol Methods. 1987 Jun 26;100(1-2):161-72 PMID: 3298436
  12. Measurement of monoclonal antibody affinity by non-competitive enzyme immunoassay.
    J Immunol Methods. 1987 Jun 26;100(1-2):173-9 PMID: 2439600
  13. Regional localization of a glioma-associated antigen defined by monoclonal antibody 81C6 in vivo: kinetics and implications for diagnosis and therapy.
    Cancer Res. 1987 Aug 15;47(16):4432-43 PMID: 3607773
  14. Biodistribution of monoclonal IgG1, F(ab')2, and Fab' in mice after intravenous injection. Comparison between anti-B cell (anti-Lyb8.2) and irrelevant (MOPC-21) antibodies.
    J Immunol. 1987 Nov 1;139(9):3041-9 PMID: 3499463
  15. In vitro measurement of avidity of radioiodinated antibodies.
    Int J Rad Appl Instrum B. 1987;14(6):605-10 PMID: 3501420
  16. The pharmacology of monoclonal antibodies.
    Ann N Y Acad Sci. 1987;507:199-210 PMID: 3327413
  17. Uptake kinetics of monoclonal antibodies by human malignant melanoma multicell spheroids.
    Cancer Res. 1988 Apr 1;48(7):1856-63 PMID: 3349464
  18. Mathematical model of simultaneous diffusion and binding of antitumor antibodies in multicellular human tumor spheroids.
    Cancer Res. 1988 Jul 15;48(14):4032-7 PMID: 3383196
  19. Generation and characterization of B72.3 second generation monoclonal antibodies reactive with the tumor-associated glycoprotein 72 antigen.
    Cancer Res. 1988 Aug 15;48(16):4588-96 PMID: 3396010
  20. Effect of specific antibody pretreatment on liver uptake of 111In-labeled anticarcinoembryonic antigen monoclonal antibody in nude mice bearing human colon cancer xenografts.
    Cancer Res. 1989 Mar 15;49(6):1587-94 PMID: 2924309
  21. Delivery of novel therapeutic agents in tumors: physiological barriers and strategies.
    J Natl Cancer Inst. 1989 Apr 19;81(8):570-6 PMID: 2649688
  22. Effect of dose, molecular size, affinity, and protein binding on tumor uptake of antibody or ligand: a biomathematical model.
    Cancer Res. 1989 Jun 15;49(12):3290-6 PMID: 2720683
  23. Direct measurement of interstitial convection and diffusion of albumin in normal and neoplastic tissues by fluorescence photobleaching.
    Proc Natl Acad Sci U S A. 1989 Jul;86(14):5385-9 PMID: 2748592
  24. Serologic mapping and biochemical characterization of the carcinoembryonic antigen epitopes using fourteen distinct monoclonal antibodies.
    Int J Cancer. 1989 Aug 15;44(2):208-18 PMID: 2474516
  25. Modeling analysis of the global and microscopic distribution of immunoglobulin G, F(ab')2, and Fab in tumors.
    Cancer Res. 1989 Oct 15;49(20):5656-63 PMID: 2790783
  26. Improving the tumor retention of radioiodinated antibody: aryl carbohydrate adducts.
    Cancer Res. 1990 Feb 1;50(3 Suppl):783s-788s PMID: 2297724
  27. Comparison of tumor targeting in nude mice by murine monoclonal antibodies directed against different human colorectal cancer antigens.
    Cancer Res. 1990 Feb 1;50(3 Suppl):828s-834s PMID: 2297729
  28. Convection and diffusion measurements using fluorescence recovery after photobleaching and video image analysis: in vitro calibration and assessment.
    Microvasc Res. 1990 Jan;39(1):77-93 PMID: 2314308
  29. Cloning of the genes for T84.66, an antibody that has a high specificity and affinity for carcinoembryonic antigen, and expression of chimeric human/mouse T84.66 genes in myeloma and Chinese hamster ovary cells.
    Cancer Res. 1990 Apr 1;50(7):2128-34 PMID: 2107969
  30. Quality control test for immunoreactivity of radiolabeled antibody.
    Biotechniques. 1990 Jan;8(1):70-5 PMID: 2322456
  31. Radiolocalization of human small cell lung cancer and antigen-positive normal tissues using monoclonal antibody LS2D617.
    Cancer Res. 1990 May 15;50(10):3124-30 PMID: 2159374
  32. Targeting of indium 111-labeled bivalent hapten to human melanoma mediated by bispecific monoclonal antibody conjugates: imaging of tumors hosted in nude mice.
    Cancer Res. 1990 Jun 1;50(11):3445-52 PMID: 2334941
  33. The kinetics of carbohydrate binding to concanavalin A.
    J Biol Chem. 1973 Nov 10;248(21):7547-51 PMID: 4745780
  34. Fluorescence correlation spectroscopy. II. An experimental realization.
    Biopolymers. 1974 Jan;13(1):29-61 PMID: 4818131
  35. Evidence for lack of damage during photobleaching measurements of the lateral mobility of cell surface components.
    Exp Cell Res. 1978 Oct 1;116(1):179-89 PMID: 359340
  36. Membrane damage caused by irradiation of fluorescent concanavalin A.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3314-7 PMID: 291005
  37. Analysis of cell surface interactions by measurements of lateral mobility.
    J Supramol Struct. 1979;12(4):481-9 PMID: 398911
  38. Measuring surface dynamics of biomolecules by total internal reflection fluorescence with photobleaching recovery or correlation spectroscopy.
    Biophys J. 1981 Mar;33(3):435-54 PMID: 7225515
  39. Total internal reflection/fluorescence photobleaching recovery study of serum albumin adsorption dynamics.
    Biophys J. 1981 Mar;33(3):455-67 PMID: 7194696
  40. Optimal strategies in immunology III. The IgM-IgG switch.
    J Math Biol. 1980 Nov;10(3):209-56 PMID: 7252371
  41. A spectrum of monoclonal antibodies reactive with human mammary tumor cells.
    Proc Natl Acad Sci U S A. 1981 May;78(5):3199-203 PMID: 6789331
  42. Mechanism of binding of multivalent immune complexes to Fc receptors. 1. Equilibrium binding.
    Biochemistry. 1981 Oct 27;20(22):6326-34 PMID: 7306512
  43. Association dynamics and lateral transport in biological membranes.
    J Supramol Struct Cell Biochem. 1981;17(1):61-7 PMID: 7321054
  44. Pharmacokinetic analysis of immunotoxin uptake in solid tumors: role of plasma kinetics, capillary permeability, and binding.
    Cancer Res. 1990 Nov 15;50(22):7382-92 PMID: 2224866
  45. Quantification of transport and binding parameters using fluorescence recovery after photobleaching. Potential for in vivo applications.
    Biophys J. 1990 Oct;58(4):873-85 PMID: 2248992
  46. Binding of a monoclonal antibody and its Fab fragment to supported phospholipid monolayers measured by total internal reflection fluorescence microscopy.
    Biophys J. 1990 Nov;58(5):1235-49 PMID: 2291943
  47. Transport of fluid and macromolecules in tumors. III. Role of binding and metabolism.
    Microvasc Res. 1991 Jan;41(1):5-23 PMID: 2051954
  48. Transport of fluid and macromolecules in tumors. IV. A microscopic model of the perivascular distribution.
    Microvasc Res. 1991 Mar;41(2):252-72 PMID: 2051960
  49. Fluorescence correlation spectroscopy and photobleaching recovery of multiple binding reactions. II. FPR and FCS measurements at low and high DNA concentrations.
    Biopolymers. 1983 Aug;22(8):1949-66 PMID: 6616015
  50. The interaction of monoclonal antibodies with MHC class I antigens on mouse spleen cells. I. Analysis of the mechanism of binding.
    J Immunol. 1984 Feb;132(2):751-8 PMID: 6690617
  51. Fluorescence correlation spectroscopy and photobleaching recovery of multiple binding reactions. I. Theory and FCS measurements.
    Biopolymers. 1983 Aug;22(8):1919-48 PMID: 6616014
Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(91)82089-6
Published
1991-09-00
Pages
596-610
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1260103
Subset
IM
Grants
NCI NIH HHS · CA-36902 · United States
NCI NIH HHS · CA-49742 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(91)82089-6 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2