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PMID: 19299621 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Ankyrin-G promotes cyclic nucleotide-gated channel transport to rod photoreceptor sensory cilia.

Science (New York, N.Y.) ·Vol. 323 ·No. 5921 ·2009-03-20 ·Pages 1614-7

Kizhatil K, Baker SA, Arshavsky VY, Bennett V

Abstract

Cyclic nucleotide-gated (CNG) channels localize exclusively to the plasma membrane of photosensitive outer segments of rod photoreceptors where they generate the electrical response to light. Here, we report the finding that targeting of CNG channels to the rod outer segment required their interaction with ankyrin-G. Ankyrin-G localized exclusively to rod outer segments, coimmunoprecipitated with the CNG channel, and bound to the C-terminal domain of the channel beta1 subunit. Ankyrin-G depletion in neonatal mouse retinas markedly reduced CNG channel expression. Transgenic expression of CNG channel beta-subunit mutants in Xenopus rods showed that ankyrin-G binding was necessary and sufficient for targeting of the beta1 subunit to outer segments. Thus, ankyrin-G is required for transport of CNG channels to the plasma membrane of rod outer segments.

MeSH Terms
Amino Acid Sequence Animals Animals, Genetically Modified Ankyrins/metabolism Cattle Cell Line Cell Membrane/metabolism Cilia/metabolism Cyclic Nucleotide-Gated Cation Channels/metabolism Humans Mice Molecular Sequence Data Nerve Tissue Proteins/metabolism Recombinant Fusion Proteins/metabolism Rod Cell Outer Segment/metabolism Xenopus laevis
Chemicals
Ank3 protein, mouse Ankyrins CNGB1 protein, human Cngb1 protein, mouse Cyclic Nucleotide-Gated Cation Channels Nerve Tissue Proteins Recombinant Fusion Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kizhatil Krishnakumar
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.
Baker Sheila A
Arshavsky Vadim Y
Bennett Vann
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
DOI
10.1126/science.1169789
Published
2009-03-20
Pages
1614-7
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC2792576
Subset
IM
Grants
NEI NIH HHS · P30 EY005722-23 · United States
NEI NIH HHS · R01 EY012859-10 · United States
Howard Hughes Medical Institute · United States
NEI NIH HHS · EY12859 · United States
External Links
PubMed source DOI: 10.1126/science.1169789 PMC Full Text View journal details
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