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PMID: 19290053 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human TRIM gene expression in response to interferons.

PloS one ·Vol. 4 ·No. 3 ·2009-00-00 ·Pages e4894

Carthagena L, Bergamaschi A, Luna JM, David A, Uchil PD, Margottin-Goguet F, Mothes W, Hazan U, Transy C, Pancino G, Nisole S

Abstract

Tripartite motif (TRIM) proteins constitute a family of proteins that share a conserved tripartite architecture. The recent discovery of the anti-HIV activity of TRIM5alpha in primate cells has stimulated much interest in the potential role of TRIM proteins in antiviral activities and innate immunity. To test if TRIM genes are up-regulated during antiviral immune responses, we performed a systematic analysis of TRIM gene expression in human primary lymphocytes and monocyte-derived macrophages in response to interferons (IFNs, type I and II) or following FcgammaR-mediated activation of macrophages. We found that 27 of the 72 human TRIM genes are sensitive to IFN. Our analysis identifies 9 additional TRIM genes that are up-regulated by IFNs, among which only 3 have previously been found to display an antiviral activity. Also, we found 2 TRIM proteins, TRIM9 and 54, to be specifically up-regulated in FcgammaR-activated macrophages. Our results present the first comprehensive TRIM gene expression analysis in primary human immune cells, and suggest the involvement of additional TRIM proteins in regulating host antiviral activities.

MeSH Terms
Adaptor Proteins, Signal Transducing/genetics Gene Expression Regulation/drug effects Humans Interferons/pharmacology Macrophages/drug effects,metabolism Membrane Proteins/genetics Phylogeny Promoter Regions, Genetic Reverse Transcriptase Polymerase Chain Reaction
Chemicals
Adaptor Proteins, Signal Transducing Membrane Proteins TRAT1 protein, human Interferons
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Carthagena Laetitia
Département des Maladies Infectieuses, Institut Cochin, Université Paris Descartes, CNRS, UMR 8104, Paris, France.
Bergamaschi Anna
Luna Joseph M
David Annie
Uchil Pradeep D
Margottin-Goguet Florence
Mothes Walther
Hazan Uriel
Transy Catherine
Pancino Gianfranco
Nisole Sébastien
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0004894
Published
2009-00-00
Epub
2009-00-17
Pages
e4894
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC2654144
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0004894 PMC Full Text View journal details
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