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PMID: 19279104 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The LMP1 promoter can be transactivated directly by NF-kappaB.

Journal of virology ·Vol. 83 ·No. 10 ·2009-05-00 ·Pages 5269-77

Demetriades C, Mosialos G

Abstract

A bioinformatic analysis identified two putative NF-kappaB binding sites in the Epstein-Barr virus (EBV) latent membrane protein 1 (LMP1) promoter. The ability of p65RelA to interact with the LMP1 promoter was shown by in vitro and in vivo assays. Using an EBV-transformed lymphoblastoid cell line as a reporter system for the activity of the +40/-328 LMP1 promoter region, the functional importance of NF-kappaB and other transcription factor binding sites was demonstrated. p65RelA could also induce LMP1 expression from the EBV genome in Daudi and P3HR1 Burkitt's lymphoma cell lines. Finally, it was shown that p65RelA could cooperate with EBNA2 or the aryl hydrocarbon receptor in the transactivation of the LMP1 promoter. Our study established the importance of NF-kappaB and several cis-acting elements in the regulation of the LMP1 promoter in a latency III environment and highlighted a complex interplay between NF-kappaB and other transcription factors in this process.

MeSH Terms
Base Sequence Binding Sites Cell Line, Transformed Gene Expression Regulation, Viral Herpesvirus 4, Human/genetics,metabolism Humans Molecular Sequence Data Promoter Regions, Genetic Protein Binding Transcription Factor RelA/metabolism Transcriptional Activation Viral Matrix Proteins/genetics
Chemicals
EBV-associated membrane antigen, Epstein-Barr virus Transcription Factor RelA Viral Matrix Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Demetriades Constantinos
School of Biology, Aristotle University of Thessaloniki, University Campus, 54124 Thessaloniki, Greece.
Mosialos George
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
1098-5514
DOI
10.1128/JVI.00097-09
Published
2009-05-00
Epub
2009-00-11
Pages
5269-77
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC2682074
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.00097-09 PMC Full Text View journal details
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