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PMID: 19270702 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Distinct transcriptional outputs associated with mono- and dimethylated histone H3 arginine 2.

Nature structural & molecular biology ·Vol. 16 ·No. 4 ·2009-04-00 ·Pages 449-51

Kirmizis A, Santos-Rosa H, Penkett CJ, Singer MA, Green RD, Kouzarides T

Abstract

Dimethylation of histone H3 Arg2 (H3R2me2) maintains transcriptional silencing by inhibiting Set1 mediated trimethylation of H3K4. Here we demonstrate that Arg2 is also monomethylated (H3R2me1) in yeast but that its functional characteristics are distinct from H3R2me2: (i) H3R2me1 does not inhibit histone H3 Lys4 (H3K4) methylation; (ii) it is present throughout the coding region of genes; and (iii) it correlates with active transcription. Collectively, these results indicate that different H3R2 methylation states have defined roles in gene expression.

MeSH Terms

Arginine/metabolism Gene Expression Regulation, Fungal Histones/metabolism Methylation Saccharomyces cerevisiae/physiology

Chemicals

Histones Arginine

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Kirmizis Antonis

Gurdon Institute and Department of Pathology, Cambridge CB2 1QN, UK.

Santos-Rosa Helena

Penkett Christopher J

Singer Michael A

Green Roland D

Kouzarides Tony

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14 references, click to expand

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Article Info

Journal

Nature structural & molecular biology

Abbr.

Nat Struct Mol Biol

ISSN

1545-9985

DOI

10.1038/nsmb.1569

Published

2009-04-00

Epub

2009-00-08

Pages

449-51

Language

English

Region

United States

NLM ID

101186374

PMCID

PMC3350867

Subset

Grants

Wellcome Trust · 092096 · United Kingdom

Cancer Research UK · United Kingdom

Databases

GEO

GSE14453

External Links

PubMed source DOI: 10.1038/nsmb.1569 PMC Full Text View journal details

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Distinct transcriptional outputs associated with mono- and dimethylated histone H3 arginine 2.

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