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PMID: 19254531 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Stochastic modeling of calcium in 3D geometry.

Biophysical journal ·Vol. 96 ·No. 5 ·2009-03-04 ·Pages 1691-706

Mazel T, Raymond R, Raymond-Stintz M, Jett S, Wilson BS

Abstract

Release of inflammatory mediators by mast cells in type 1 immediate-hypersensitivity allergic reactions relies on antigen-dependent increases in cytosolic calcium. Here, we used a series of electron microscopy images to build a 3D reconstruction representing a slice through a rat tumor mast cell, which then served as a basis for stochastic modeling of inositol-trisphosphate-mediated calcium responses. The stochastic approach was verified by reaction-diffusion modeling within the same geometry. Local proximity of the endoplasmic reticulum to either the plasma membrane or mitochondria is predicted to differentially impact local inositol trisphosphate receptor transport. The explicit consideration of organelle spatial relationships represents an important step toward building a comprehensive, realistic model of cellular calcium dynamics.

MeSH Terms
Animals Calcium/metabolism Cell Line, Tumor Cell Membrane/physiology Computer Simulation Diffusion Endoplasmic Reticulum/physiology Imaging, Three-Dimensional Inositol 1,4,5-Trisphosphate Receptors/metabolism Inositol Phosphates/metabolism Leukemia, Basophilic, Acute/metabolism Mast Cells/metabolism,ultrastructure Microscopy, Electron Mitochondria/physiology Models, Biological Rats Stochastic Processes
Chemicals
Inositol 1,4,5-Trisphosphate Receptors Inositol Phosphates inositol trispyrophosphate Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mazel Tomás
Department of Pathology and Cancer Research and Treatment Center, University of New Mexico School of Medicine, Albuquerque, New Mexico, USA.
Raymond Rebecca
Raymond-Stintz Mary
Jett Stephen
Wilson Bridget S
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
1542-0086
DOI
10.1016/j.bpj.2008.10.066
Published
2009-03-04
Pages
1691-706
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC2996128
Subset
IM
Grants
NIGMS NIH HHS · P50 GM085273 · United States
NIAID NIH HHS · R01 AI051575 · United States
NIAID NIH HHS · R01 AI051575-06A2 · United States
FDA HHS · P20 BM0066283 · United States
External Links
PubMed source DOI: 10.1016/j.bpj.2008.10.066 PMC Full Text View journal details
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