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PMID: 19249230 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Cytokine-producing effector B cells regulate type 2 immunity to H. polygyrus.

Immunity ·Vol. 30 ·No. 3 ·2009-03-20 ·Pages 421-33

Wojciechowski W, Harris DP, Sprague F, Mousseau B, Makris M, Kusser K, Honjo T, Mohrs K, Mohrs M, Randall T, Lund FE

Abstract

Immunity to the intestinal parasite Heligomosomoides polygyrus is dependent on the successful generation of T helper 2 (Th2) memory cells. We showed that B cells contribute to immunity against H. polygyrus by producing antibody (Ab) and by promoting expansion and differentiation of primary and memory Th2 cells. We also demonstrated that cytokine-producing effector B cells were essential for effective immunity to H. polygyrus. Tumor necrosis factor alpha production by B cells was necessary for sustained Ab production, whereas interleukin 2 production by B cells was necessary for Th2 cell expansion and differentiation. These results show that B cells mediate protection from pathogens not only by presenting antigen and secreting antibody but also by producing cytokines that regulate the quality and magnitude of humoral and cellular immune responses.

MeSH Terms
Animals Antibody Formation Antigens, Helminth/immunology B-Lymphocytes/immunology Cytokines/metabolism Enzyme-Linked Immunosorbent Assay Immunity, Cellular Interleukin-4/metabolism Mice Mice, Inbred BALB C Mice, Inbred C57BL Nematospiroides dubius/immunology Strongylida Infections/immunology,prevention & control Th2 Cells/immunology
Chemicals
Antigens, Helminth Cytokines Interleukin-4
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Wojciechowski Wojciech
Trudeau Institute, Saranac Lake, NY 12983, USA.
Harris David P
Sprague Frank
Mousseau Betty
Makris Melissa
Kusser Kim
Honjo Tasuko
Mohrs Katja
Mohrs Markus
Randall Troy
Lund Frances E
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Article Info
Journal
Immunity
Abbr.
Immunity
ISSN
1097-4180
DOI
10.1016/j.immuni.2009.01.006
Published
2009-03-20
Epub
2009-00-26
Pages
421-33
Language
English
Region
United States
NLM ID
9432918
PMCID
PMC2745290
Subset
IM
Grants
NIAID NIH HHS · R01 AI068056-03 · United States
NIAID NIH HHS · AI-68056 · United States
NIAID NIH HHS · R01 AI068056-04 · United States
NIAID NIH HHS · R01 AI068056-05 · United States
NIAID NIH HHS · AI-50844 · United States
NIAID NIH HHS · R01 AI068056 · United States
NIAID NIH HHS · R01 AI050844 · United States
Corrections
CommentIn
PMID: 19303384
External Links
PubMed source DOI: 10.1016/j.immuni.2009.01.006 PMC Full Text View journal details
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