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PMID: 1924559 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

New shuttle vectors for Haemophilus influenzae and Escherichia coli: P15A-derived plasmids replicate in H. influenzae Rd.

Plasmid ·Vol. 25 ·No. 3 ·1991-05-00 ·Pages 221-4

Chandler MS

Abstract

With the aim of identifying new plasmids useful for molecular cloning in Haemophilus influenzae, several P15A-derived plasmids were tested for their ability to transform H. influenzae Rd. The four plasmids tested, pACYC177, pACYC184, pSU2718, and pSU2719 were all able to establish in H. influenzae Rd. The plasmids were stable, could be purified by standard protocols, and were compatible with a plasmid carrying the RSF0885 origin of replication.

MeSH Terms

Cloning, Molecular/methods DNA Replication Escherichia coli/genetics Genetic Vectors Haemophilus influenzae/genetics Plasmids Transformation, Bacterial

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Chandler M S

Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

Article Info

Journal

Plasmid

Abbr.

Plasmid

ISSN

0147-619X

DOI

10.1016/0147-619x(91)90016-p

Published

1991-05-00

Pages

221-4

Language

English

Region

United States

NLM ID

7802221

Subset

Grants

NCI NIH HHS · 5-T32-CA09139 · United States

Databases

GENBANK

M64731, M64732, X54193, X54314, X55687, X55688, X55689, X55690, X56856, X58180

External Links

PubMed source DOI: 10.1016/0147-619x(91)90016-p View journal details

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New shuttle vectors for Haemophilus influenzae and Escherichia coli: P15A-derived plasmids replicate in H. influenzae Rd.

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