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PMID: 19171305 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Visualization of Src activity at different compartments of the plasma membrane by FRET imaging.

Chemistry & biology ·Vol. 16 ·No. 1 ·2009-01-30 ·Pages 48-57

Seong J, Lu S, Ouyang M, Huang H, Zhang J, Frame MC, Wang Y

Abstract

Membrane compartments function as segregated signaling platforms for different cellular functions. It is not clear how Src is regulated at different membrane compartments. To visualize local Src activity in live cells, a FRET-based Src biosensor was targeted in or outside of lipid rafts at the plasma membrane, via acylation or prenylation modifications on targeting tags either directly fused to the biosensor or coupled to the biosensor through an inducible heterodimerization system. In response to growth factors and pervanadate, the induction of Src activity in rafts was slower and weaker, dependent on actin and possibly its mediated transportation of Src from perinuclear regions to the plasma membrane. In contrast, the induction of Src activity in nonrafts was faster and stronger, dependent on microtubules. Hence, Src activity is differentially regulated via cytoskeleton at different membrane compartments.

MeSH Terms
Actins/metabolism Biosensing Techniques Cell Membrane/enzymology Cells, Cultured Cytoskeleton/metabolism Epidermal Growth Factor/metabolism Fluorescence Resonance Energy Transfer/methods HeLa Cells Humans Microtubules/metabolism Platelet-Derived Growth Factor/pharmacology Protein Transport Vanadates/pharmacology src-Family Kinases/antagonists & inhibitors,metabolism
Chemicals
Actins Platelet-Derived Growth Factor pervanadate Vanadates Epidermal Growth Factor src-Family Kinases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Seong Jihye
Neuroscience Program, Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Lu Shaoying
Ouyang Mingxing
Huang He
Zhang Jin
Frame Margaret C
Wang Yingxiao
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Article Info
Journal
Chemistry & biology
Abbr.
Chem Biol
ISSN
1879-1301
DOI
10.1016/j.chembiol.2008.11.007
Published
2009-01-30
Pages
48-57
Language
English
Region
United States
NLM ID
9500160
PMCID
PMC2647969
Subset
IM
Grants
NIDDK NIH HHS · R01 DK073368 · United States
NCI NIH HHS · CA122673 · United States
NCI NIH HHS · R21 CA122673 · United States
NIDDK NIH HHS · DK073368 · United States
NINDS NIH HHS · R01 NS063405-01 · United States
External Links
PubMed source DOI: 10.1016/j.chembiol.2008.11.007 PMC Full Text
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