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PMID: 1916898 Published · ppublish English Journal Article

IL-8 causes in vivo neutrophil migration by a cell-dependent mechanism.

Immunology ·Vol. 73 ·No. 4 ·1991-08-00 ·Pages 472-7

Ribeiro RA, Flores CA, Cunha FQ, Ferreira SH

Abstract

A dose-dependent neutrophil migration was observed following the injection of recombinant interleukin-8 (rIL-8) into rat peritoneal cavities. This finding contrasts with the inability of rIL-8 to induce neutrophil emigration into subcutaneous air-pouches. Pre-treatment of the animals with dexamethasone (0.5 mg/kg, s.c.) or depletion of the peritoneal resident cell population abolished the neutrophil migration induced by rIL-8 and by recombinant interleukin-1 beta (rIL-1 beta). Different from that which occurs with rIL-1 beta, neutrophil migration induced by rIL-8 was not enhanced by an increase in the peritoneal macrophage population. Transference of homologous total resident peritoneal cells to the air-pouch rendered this cavity responsive to the chemotactic effect of rIL-8 and potentiated the neutrophil migration induced by rIL-1. Our results show that both rIL-8 and rIL-1 beta are able to induce in vivo neutrophil migration by an indirect mechanism, dependent on resident cells. Neither macrophages nor lymphocytes seem to be involved in the rIL-8 chemotactic effect. However, peritoneal resident mast cells may be implicated in this mechanism. These cells, when stimulated in vitro by rIL-8, released a factor that when injected into peritoneal and air-pouch cavities induced neutrophil migration.

MeSH Terms
Animals Chemotaxis, Leukocyte/drug effects,immunology Dexamethasone/pharmacology Interleukin-1/immunology Interleukin-8/immunology Male Mast Cells/immunology Neutrophils/immunology Peritoneal Cavity/cytology Peritoneal Lavage Rats Rats, Inbred Strains Recombinant Proteins/immunology Thioglycolates/pharmacology
Chemicals
Interleukin-1 Interleukin-8 Recombinant Proteins Thioglycolates Dexamethasone
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ribeiro R A
Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Brasil.
Flores C A
Cunha F Q
Ferreira S H
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1991-08-00
Pages
472-7
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1384579
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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