Chromatin-like structures obtained after alkaline disruption of bovine and human papillomaviruses.

Journal of virology ·Vol. 21 ·No. 3 ·1977-03-00 ·Pages 1205-9

Favre M, Breitburd F, Croissant O, Orth G

Abstract

Four low-molecular-weight polypeptides migrating like H2a, H2b, H3, and H4 calf liver histones were detected by sodium dodecyl sulfate-acrylamide gel electrophoresis of highly purified preparations of bovine papillomavirus (BPV) and human papillomavirus (HPV). Complexes of these polypeptides and viral DNA were isolated by agarose-gel filtration of the alkaline disruption products of both viruses. When observed under the electron microscope, these complexes appeared as circular structures composed of nucleosomes with a diameter of about 8.0 nm interconnected by a naked DNA filament. The maximal frequency of nucleosomes per molecule was 30 for both viruses, corresponding to a condensation ratio of the viral DNA of 2.5.

MeSH Terms

Bovine papillomavirus 1/analysis Chromatin/analysis DNA, Viral/analysis Electrophoresis, Polyacrylamide Gel Histones/analysis Microscopy, Electron Papillomaviridae/analysis Viral Proteins/analysis

Chemicals

Chromatin DNA, Viral Histones Viral Proteins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Favre M

Breitburd F

Croissant O

Orth G

References (15)

15 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.21.3.1205-1209.1977

Published

1977-03-00

Pages

1205-9

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC515661

Subset

External Links

PubMed source DOI: 10.1128/JVI.21.3.1205-1209.1977 PMC Full Text View journal details

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Chromatin-like structures obtained after alkaline disruption of bovine and human papillomaviruses.

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