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PMID: 19129480 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Essential role of hIST1 in cytokinesis.

Molecular biology of the cell ·Vol. 20 ·No. 5 ·2009-03-00 ·Pages 1374-87

Agromayor M, Carlton JG, Phelan JP, Matthews DR, Carlin LM, Ameer-Beg S, Bowers K, Martin-Serrano J

Abstract

The last steps of multivesicular body (MVB) formation, human immunodeficiency virus (HIV)-1 budding and cytokinesis require a functional endosomal sorting complex required for transport (ESCRT) machinery to facilitate topologically equivalent membrane fission events. Increased sodium tolerance (IST) 1, a new positive modulator of the ESCRT pathway, has been described recently, but an essential function of this highly conserved protein has not been identified. Here, we describe the previously uncharacterized KIAA0174 as the human homologue of IST1 (hIST1), and we report its conserved interaction with VPS4, CHMP1A/B, and LIP5. We also identify a microtubule interacting and transport (MIT) domain interacting motif (MIM) in hIST1 that is necessary for its interaction with VPS4, LIP5 and other MIT domain-containing proteins, namely, MITD1, AMSH, UBPY, and Spastin. Importantly, hIST1 is essential for cytokinesis in mammalian cells but not for HIV-1 budding, thus providing a novel mechanism of functional diversification of the ESCRT machinery. Last, we show that the hIST1 MIM activity is essential for cytokinesis, suggesting possible mechanisms to explain the role of hIST1 in the last step of mammalian cell division.

MeSH Terms
Amino Acid Sequence Binding Sites Carrier Proteins/metabolism Cytokinesis/physiology Endosomal Sorting Complexes Required for Transport Endosomes/metabolism HIV-1/physiology HeLa Cells Humans Molecular Sequence Data Nuclear Proteins/metabolism Oncogene Proteins/analysis,chemistry,physiology Protein Interaction Mapping Protein Structure, Tertiary Protein Transport/genetics Sequence Alignment Vesicular Transport Proteins
Chemicals
CHMP1A protein, human Carrier Proteins Endosomal Sorting Complexes Required for Transport IST1 protein, human Nuclear Proteins Oncogene Proteins VTA1 protein, human Vesicular Transport Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Agromayor Monica
Department of Infectious Diseases, King's College London School of Medicine, London, United Kingdom.
Carlton Jez G
Phelan John P
Matthews Daniel R
Carlin Leo M
Ameer-Beg Simon
Bowers Katherine
Martin-Serrano Juan
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1939-4586
DOI
10.1091/mbc.e08-05-0474
Published
2009-03-00
Epub
2009-00-07
Pages
1374-87
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC2649264
Subset
IM
Grants
Medical Research Council · G0601097 · United Kingdom
Medical Research Council · G0400207 · United Kingdom
External Links
PubMed source DOI: 10.1091/mbc.e08-05-0474 PMC Full Text View journal details
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