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PMID: 19129445 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sindbis virus-based measles DNA vaccines protect cotton rats against respiratory measles: relevance of antibodies, mucosal and systemic antibody-secreting cells, memory B cells, and Th1-type cytokines as correlates of immunity.

Journal of virology ·Vol. 83 ·No. 6 ·2009-03-00 ·Pages 2789-94

Pasetti MF, Ramirez K, Resendiz-Albor A, Ulmer J, Barry EM, Levine MM

Abstract

Measles remains an important cause of pediatric morbidity and mortality in developing countries, especially among infants who are too young to receive the current licensed live attenuated measles vaccine. We developed two Sindbis virus DNA vaccines encoding the measles virus hemagglutinin (pMSIN-H) and fusion proteins (pMSINH-FdU) and examined their immunogenicities and protective efficacies when administered alone or followed by the live measles virus vaccine in cotton rats. Neutralizing antibodies, mucosal and systemic antibody-secreting cells, memory B cells, and gamma interferon-secreting T cells developed after priming and increased after boosting. pMSIN-H priming conferred 100% protection against pulmonary measles, whereas pMSINH-FdU protected only in conjunction with the live measles virus vaccine boost.

MeSH Terms

Animals Antibodies, Viral/blood,immunology Antibody-Producing Cells/immunology Cytokines/immunology Hemagglutinins, Viral/genetics,immunology Humans Immunity, Mucosal Immunization, Secondary Immunologic Memory Interferon-gamma/metabolism Lung/immunology,virology Measles/prevention & control Measles Vaccine/genetics,immunology Measles virus/genetics,immunology Neutralization Tests Rats Sigmodontinae Sindbis Virus/genetics Vaccines, DNA/genetics,immunology Viral Fusion Proteins/genetics,immunology

Chemicals

Antibodies, Viral Cytokines Hemagglutinins, Viral Measles Vaccine Vaccines, DNA Viral Fusion Proteins hemagglutinin protein G, measles virus Interferon-gamma

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Pasetti Marcela F

Center for Vaccine Development, University of Maryland School of Medicine, 685 West Baltimore St., Room 480, Baltimore, Maryland 21201, USA. mpasetti@medicine.umaryland.edu

Ramirez Karina

Resendiz-Albor Aldo

Ulmer Jeffrey

Barry Eileen M

Levine Myron M

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

1098-5514

DOI

10.1128/JVI.02191-08

mpasetti@medicine.umaryland.edu

Published

2009-03-00

Epub

2009-00-07

Pages

2789-94

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC2648279

Subset

External Links

PubMed source DOI: 10.1128/JVI.02191-08 PMC Full Text View journal details

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Sindbis virus-based measles DNA vaccines protect cotton rats against respiratory measles: relevance of antibodies, mucosal and systemic antibody-secreting cells, memory B cells, and Th1-type cytokines as correlates of immunity.

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