Superhelix densities of circular DNA's: a generalized equation for their determination by the bouyant method.

Science (New York, N.Y.) ·Vol. 195 ·No. 4281 ·1977-03-04 ·Pages 891

Upholt WB

Abstract

Equations for the measurement of the supertwisting of circular DNA's from banding positions in buoyant density gradients containing intercalating dyes have required the use of SV40 DNA isolated from virions as a reference DNA. These equations are modified to allow the use of any closed circular DNA of known superhelix density as a reference DNA.

MeSH Terms

Centrifugation, Density Gradient/methods DNA, Circular DNA, Viral Models, Biological Nucleic Acid Conformation Simian virus 40/ultrastructure

Chemicals

DNA, Circular DNA, Viral

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Upholt W B

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.190680

Published

1977-03-04

Pages

891

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.190680 View journal details

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Superhelix densities of circular DNA's: a generalized equation for their determination by the bouyant method.

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