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PMID: 19060900 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Cortical sinus probing, S1P1-dependent entry and flow-based capture of egressing T cells.

Nature immunology ·Vol. 10 ·No. 1 ·2009-01-00 ·Pages 58-65

Grigorova IL, Schwab SR, Phan TG, Pham TH, Okada T, Cyster JG

Abstract

The cellular dynamics of the egress of lymphocytes from lymph nodes are poorly defined. Here we visualized the branched organization of lymph node cortical sinuses and found that after entry, some T cells were retained, whereas others returned to the parenchyma. T cells deficient in sphingosine 1-phosphate receptor type 1 probed the sinus surface but failed to enter the sinuses. In some sinuses, T cells became rounded and moved unidirectionally. T cells traveled from cortical sinuses into macrophage-rich sinus areas. Many T cells flowed from medullary sinuses into the subcapsular space. We propose a multistep model of lymph node egress in which cortical sinus probing is followed by entry dependent on sphingosine 1-phosphate receptor type 1, capture of cells in a sinus region with flow, and transport to medullary sinuses and the efferent lymph.

MeSH Terms
Animals Cell Movement/genetics Germinal Center/immunology Glycoproteins/immunology Homeodomain Proteins/genetics Lymph Nodes/immunology Lymphatic System Membrane Transport Proteins Mice Mice, Congenic Mice, Inbred C57BL Receptors, Lysosphingolipid/genetics,physiology T-Lymphocytes/immunology
Chemicals
Glycoproteins Homeodomain Proteins Membrane Transport Proteins Receptors, Lysosphingolipid Xlkd1 protein, mouse RAG-1 protein
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Grigorova Irina L
Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California 94143, USA.
Schwab Susan R
Phan Tri Giang
Pham Trung H M
Okada Takaharu
Cyster Jason G
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Article Info
Journal
Nature immunology
Abbr.
Nat Immunol
ISSN
1529-2916
DOI
10.1038/ni.1682
Published
2009-01-00
Epub
2008-00-07
Pages
58-65
Language
English
Region
United States
NLM ID
100941354
PMCID
PMC2710451
Subset
IM
Grants
NIAID NIH HHS · R01 AI074847-02 · United States
NIAID NIH HHS · R01 AI045073-10 · United States
NIAID NIH HHS · R01 AI045073-08 · United States
NIAID NIH HHS · R01 AI074847-01 · United States
NIAID NIH HHS · R01 AI045073-09 · United States
NIAID NIH HHS · R01 AI074847 · United States
Howard Hughes Medical Institute · United States
NIAID NIH HHS · R01 AI045073 · United States
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1038/ni.1682 PMC Full Text View journal details
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