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题目:: B cell helper assays.
作者:: Abrignani(Sergio),Tonti(Elena),Casorati(Giulia),Dellabona(Paolo)
状态:: 发布时间2008-12-02 , 更新时间 2008-12-02
期刊:: Methods Mol Biol
摘要:: Activation, proliferation and differentiation of naïve B lymphocytes into memory B cells and plasma cells requires engagement of the B cell receptor (BCR) coupled to T-cell help (1, 2). T cells deliver help in cognate fashion when they are activated upon recognition of specific MHC-peptide complexes presented by B cells. T cells can also deliver help in a non-cognate or bystander fashion, when they do not find specific MHC-peptide complexes on B cells and are activated by alternative mechanisms. T-cell dependent activation of B cells can be studied in vitro by experimental models called "B cell helper assays" that are based on the co-culture of B cells with activated T cells. These assays allow to decipher the molecular bases for productive T-dependent B cell responses. We show here examples of B cell helper assays in vitro, which can be reproduced with any subset of T lymphocytes that displays the appropriate helper signals.
语言:: eng
DOI:: 10.1007/978-1-60327-527-9_2

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