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PMID: 190404 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Agonist induced release of intracellular Ca2+ in the rabbit aorta.

The Journal of membrane biology ·Vol. 30 ·No. 4 ·1977-01-28 ·Pages 363-80

Deth R, van Breemen C

Abstract

The effects of hormonal agonists (norepinephrine, angiotensin, and histamine) on 45Ca efflux from the rabbit aorta were studied using a Ca-EGTA buffered efflux medium. Each caused a transient stimulation of efflux rate which probably reflected the release of an intracellular 45Ca store. The size of the stimulation of efflux correlated with the size of the initial rapid phase of contraction. The norepinephrine-sensitive intracellular Ca fraction was estimated to be greater than 21 mumoles/Kg wet tissue weight. This fraction is separate from intracellular Ca which is accumulated during relaxation. Evidence is presented for the lack of cyclic nucleotide involvement in the release of Ca2+, and possible alternative modes of coupling are discussed.

MeSH Terms
Angiotensin II/pharmacology Animals Aorta/drug effects,metabolism Biological Transport Calcium/metabolism Cyclic GMP/analogs & derivatives,pharmacology Egtazic Acid/pharmacology Histamine/pharmacology Isoproterenol/pharmacology Kinetics Lanthanum/pharmacology Muscle Contraction Muscle, Smooth Norepinephrine/pharmacology Phenoxybenzamine/pharmacology Rabbits
Chemicals
Phenoxybenzamine Angiotensin II Egtazic Acid Lanthanum Histamine Cyclic GMP Isoproterenol Calcium Norepinephrine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Deth R
van Breemen C
References (34)
34 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01869677
Published
1977-01-28
Pages
363-80
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01869677 View journal details
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