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PMID: 19033538 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

T cell transfer model of chronic colitis: concepts, considerations, and tricks of the trade.

American journal of physiology. Gastrointestinal and liver physiology ·Vol. 296 ·No. 2 ·2009-02-00 ·Pages G135-46

Ostanin DV, Bao J, Koboziev I, Gray L, Robinson-Jackson SA, Kosloski-Davidson M, Price VH, Grisham MB

Abstract

The inflammatory bowel diseases (Crohn's disease; ulcerative colitis) are idiopathic chronic inflammatory disorders of the intestine and/or colon. A major advancement in our understanding of the pathogenesis of these diseases has been the development of mouse models of chronic gut inflammation. One model that has been instrumental in delineating the immunological mechanisms responsible for the induction as well as regulation of intestinal inflammation is the T cell transfer model of chronic colitis. This paper presents a detailed protocol describing the methods used to induce chronic colitis in mice. Special attention is given to the immunological concepts that explain disease pathogenesis in this model, considerations and potential pitfalls in using this model, and finally different "tricks" that we have learned over the past 12 years that have allowed us to develop a more simplified version of this model of experimental IBD.

MeSH Terms
Adoptive Transfer Animals CD11a Antigen/genetics,metabolism CD4-Positive T-Lymphocytes/immunology,transplantation Cell Separation Cells, Cultured Chronic Disease Colitis/immunology,pathology Colon/immunology,pathology Disease Models, Animal Disease Progression Flow Cytometry Homeodomain Proteins/genetics,metabolism Inflammatory Bowel Diseases/immunology,pathology Interleukin-10/genetics,metabolism Leukocyte Common Antigens/analysis Mice Mice, Inbred C57BL Mice, Knockout Severity of Illness Index T-Lymphocytes, Regulatory/immunology,transplantation Time Factors
Chemicals
CD11a Antigen Homeodomain Proteins RAG-1 protein Interleukin-10 Leukocyte Common Antigens Ptprc protein, mouse
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ostanin Dmitry V
Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, LA 71130-3932, USA.
Bao Jianxiong
Koboziev Iurii
Gray Laura
Robinson-Jackson Sherry A
Kosloski-Davidson Melissa
Price V Hugh
Grisham Matthew B
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Article Info
Journal
American journal of physiology. Gastrointestinal and liver physiology
Abbr.
Am J Physiol Gastrointest Liver Physiol
ISSN
0193-1857
DOI
10.1152/ajpgi.90462.2008
Published
2009-02-00
Epub
2008-00-25
Pages
G135-46
Language
English
Region
United States
NLM ID
100901227
PMCID
PMC2643911
Subset
IM
Grants
NIDDK NIH HHS · P01 DK-43785 · United States
External Links
PubMed source DOI: 10.1152/ajpgi.90462.2008 PMC Full Text View journal details
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