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PMID: 19002256 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Validation Study

Genome-scale validation of deep-sequencing libraries.

PloS one ·Vol. 3 ·No. 11 ·2008-00-00 ·Pages e3713

Schmidt D, Stark R, Wilson MD, Brown GD, Odom DT

Abstract

Chromatin immunoprecipitation followed by high-throughput (HTP) sequencing (ChIP-seq) is a powerful tool to establish protein-DNA interactions genome-wide. The primary limitation of its broad application at present is the often-limited access to sequencers. Here we report a protocol, Mab-seq, that generates genome-scale quality evaluations for nucleic acid libraries intended for deep-sequencing. We show how commercially available genomic microarrays can be used to maximize the efficiency of library creation and quickly generate reliable preliminary data on a chromosomal scale in advance of deep sequencing. We also exploit this technique to compare enriched regions identified using microarrays with those identified by sequencing, demonstrating that they agree on a core set of clearly identified enriched regions, while characterizing the additional enriched regions identifiable using HTP sequencing.

MeSH Terms
Chromatin Immunoprecipitation Genome Genomic Library Genomics/methods Humans Models, Biological Oligonucleotide Array Sequence Analysis
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schmidt Dominic
Cancer Research UK, Cambridge Research Institute, Li Ka Shing Centre, Cambridge, UK.
Stark Rory
Wilson Michael D
Brown Gordon D
Odom Duncan T
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
Published
2008-00-00
Epub
2008-00-12
Pages
e3713
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC2577887
Subset
IM
Grants
Cancer Research UK · 15603 · United Kingdom
European Research Council · 202218 · International
Cancer Research UK · A10185 · United Kingdom
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