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PMID: 19000842 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Atlas of gene expression in the developing kidney at microanatomic resolution.

Developmental cell ·Vol. 15 ·No. 5 ·2008-11-00 ·Pages 781-91

Brunskill EW, Aronow BJ, Georgas K, Rumballe B, Valerius MT, Aronow J, Kaimal V, Jegga AG, Yu J, Grimmond S, McMahon AP, Patterson LT, Little MH, Potter SS

Abstract

Kidney development is based on differential cell-type-specific expression of a vast number of genes. While multiple critical genes and pathways have been elucidated, a genome-wide analysis of gene expression within individual cellular and anatomic structures is lacking. Accomplishing this could provide significant new insights into fundamental developmental mechanisms such as mesenchymal-epithelial transition, inductive signaling, branching morphogenesis, and segmentation. We describe here a comprehensive gene expression atlas of the developing mouse kidney based on the isolation of each major compartment by either laser capture microdissection or fluorescence-activated cell sorting, followed by microarray profiling. The resulting data agree with known expression patterns and additional in situ hybridizations. This kidney atlas allows a comprehensive analysis of the progression of gene expression states during nephrogenesis, as well as discovery of potential growth factor-receptor interactions. In addition, the results provide deeper insight into the genetic regulatory mechanisms of kidney development.

MeSH Terms
Animals Embryo, Mammalian/metabolism Gene Expression Profiling Gene Expression Regulation, Developmental Kidney/embryology,metabolism Mice Mice, Inbred Strains Nephrons/embryology,metabolism Oligonucleotide Array Sequence Analysis
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Brunskill Eric W
Division of Developmental Biology, Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA.
Aronow Bruce J
Georgas Kylie
Rumballe Bree
Valerius M Todd
Aronow Jeremy
Kaimal Vivek
Jegga Anil G
Yu Jing
Grimmond Sean
McMahon Andrew P
Patterson Larry T
Little Melissa H
Potter S Steven
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Article Info
Journal
Developmental cell
Abbr.
Dev Cell
ISSN
1878-1551
DOI
10.1016/j.devcel.2008.09.007
Published
2008-11-00
Pages
781-91
Language
English
Region
United States
NLM ID
101120028
PMCID
PMC2653061
Subset
IM
Grants
NIDDK NIH HHS · DK070136 · United States
NIDDK NIH HHS · R37 DK054364 · United States
NIDDK NIH HHS · U01 DK070251 · United States
NIDDK NIH HHS · F32 DK060319-03 · United States
NIDDK NIH HHS · U01 DK070251-03 · United States
NIDDK NIH HHS · U01 DK070136 · United States
NIDDK NIH HHS · U01 DK070181 · United States
NIDDK NIH HHS · DK070251 · United States
NIDDK NIH HHS · DK070181 · United States
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1016/j.devcel.2008.09.007 PMC Full Text View journal details
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