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PMID: 1898897 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Diphosphoryl lipid A derived from lipopolysaccharide (LPS) of Rhodopseudomonas sphaeroides inhibits activation of 70Z/3 cells by LPS.

Infection and immunity ·Vol. 59 ·No. 1 ·1991-01-00 ·Pages 131-6

Kirkland TN, Qureshi N, Takayama K

Abstract

Diphosphoryl lipid A derived from nontoxic lipopolysaccharide (LPS) of Rhodopseudomonas sphaeroides ATCC 17023 did not stimulate the murine pre-B cell line 70Z/3 to synthesize surface immunoglobulin or kappa mRNA. However, it effectively blocked Escherichia coli LPS-induced activation of 70Z/3 cells in a concentration-dependent manner. This inhibition was specific only to cells activated by LPS, since it did not inhibit activation of 70Z/3 cells by gamma interferon. Maximal inhibitory effect occurred when the antagonist was added within 2 h before adding the LPS. These results strongly suggested that R. sphaeroides diphosphoryl lipid A is competing with E. coli LPS for physiological lipid A receptors on the 70Z/3 cells.

MeSH Terms
Animals B-Lymphocytes/drug effects,immunology Hematopoietic Stem Cells/drug effects Immunoglobulin kappa-Chains/genetics Lipid A/analogs & derivatives,pharmacology Lipopolysaccharides/antagonists & inhibitors Lymphocyte Activation/drug effects Mice RNA, Messenger/analysis Receptors, Antigen, B-Cell/analysis Rhodobacter sphaeroides/metabolism Structure-Activity Relationship
Chemicals
Immunoglobulin kappa-Chains Lipid A Lipopolysaccharides RNA, Messenger Receptors, Antigen, B-Cell diphosphoryl lipid A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kirkland T N
Department of Pathology, University of California, San Diego 92093.
Qureshi N
Takayama K
References (23)
23 references, click to expand
  1. Complete structural determination of lipopolysaccharide obtained from deep rough mutant of Escherichia coli. Purification by high performance liquid chromatography and direct analysis by plasma desorption mass spectrometry.
    J Biol Chem. 1988 Aug 25;263(24):11971-6 PMID: 3136169
  2. Genetic control of responses to bacterial lipopolysaccharides in mice. II. A gene that influences a membrane component involved in the activation of bone marrow-derived lymphocytes by lipipolysaccharides.
    J Immunol. 1975 May;114(5):1462-8 PMID: 123543
  3. Induction of kappa light chain synthesis in 70Z/3 B lymphoma cells by chemically defined lipid A precursors.
    J Biol Chem. 1988 Apr 15;263(11):5098-103 PMID: 3128535
  4. I kappa B: a specific inhibitor of the NF-kappa B transcription factor.
    Science. 1988 Oct 28;242(4878):540-6 PMID: 3140380
  5. Induction of kappa transcription by interferon-gamma without activation of NF-kappa B.
    Science. 1988 Nov 18;242(4881):1036-7 PMID: 3143155
  6. Inducibility of kappa immunoglobulin enhancer-binding protein Nf-kappa B by a posttranslational mechanism.
    Cell. 1986 Dec 26;47(6):921-8 PMID: 3096580
  7. A labile inhibitor blocks immunoglobulin kappa-light-chain-gene transcription in a pre-B leukemic cell line.
    Proc Natl Acad Sci U S A. 1986 Jan;83(2):295-8 PMID: 3079910
  8. IL-1 induction-capacity of defined lipopolysaccharide partial structures.
    J Immunol. 1989 May 1;142(9):3229-38 PMID: 2651523
  9. Diphosphoryl lipid A from Rhodopseudomonas sphaeroides ATCC 17023 blocks induction of cachectin in macrophages by lipopolysaccharide.
    Infect Immun. 1989 Apr;57(4):1336-8 PMID: 2784418
  10. Inhibition of lipopolysaccharide activation of 7OZ/3 cells by anti-lipopolysaccharide antibodies.
    J Immunol. 1988 Nov 1;141(9):3208-13 PMID: 3262686
  11. Location of fatty acids in lipid A obtained from lipopolysaccharide of Rhodopseudomonas sphaeroides ATCC 17023.
    J Biol Chem. 1988 Apr 25;263(12):5502-4 PMID: 3258599
  12. Protection of mice against lethal endotoxemia by a lipid A precursor.
    Infect Immun. 1986 Jun;52(3):905-7 PMID: 3519466
  13. Inhibition of endotoxin-induced priming of human neutrophils by lipid X and 3-Aza-lipid X.
    J Clin Invest. 1987 Sep;80(3):605-12 PMID: 3624479
  14. Deacylated lipopolysaccharide inhibits neutrophil adherence to endothelium induced by lipopolysaccharide in vitro.
    J Exp Med. 1987 May 1;165(5):1393-402 PMID: 3572302
  15. Inducible transcription of the unrearranged kappa constant region locus is a common feature of pre-B cells and does not require DNA or protein synthesis.
    Proc Natl Acad Sci U S A. 1985 Aug;82(16):5305-9 PMID: 3927301
  16. Identification of lipopolysaccharide-binding proteins in 70Z/3 cells by photoaffinity cross-linking.
    J Biol Chem. 1990 Jun 5;265(16):9520-5 PMID: 2345180
  17. Nontoxic lipopolysaccharide from Rhodopseudomonas sphaeroides ATCC 17023.
    J Bacteriol. 1983 Jul;155(1):153-8 PMID: 6602801
  18. Separation and characterization of toxic and nontoxic forms of lipid A.
    Rev Infect Dis. 1984 Jul-Aug;6(4):439-43 PMID: 6474003
  19. Somatic mutation of immunoglobulin light-chain variable-region genes.
    Cell. 1981 Jul;25(1):47-58 PMID: 6791832
  20. Purification and structural determination of nontoxic lipid A obtained from the lipopolysaccharide of Salmonella typhimurium.
    J Biol Chem. 1982 Oct 10;257(19):11808-15 PMID: 6749846
  21. The synthesis and processing of the messenger RNAs specifying heavy and light chain immunoglobulins in MPC-11 cells.
    Cell. 1978 Dec;15(4):1495-509 PMID: 103631
  22. Murine B cell leukemia line with inducible surface immunoglobulin expression.
    J Immunol. 1978 Aug;121(2):641-7 PMID: 308074
  23. Location of polar substituents and fatty acyl chains on lipid A precursors from a 3-deoxy-D-manno-octulosonic acid-deficient mutant of Salmonella typhimurium. Studies by 1H, 13C, and 31P nuclear magnetic resonance.
    J Biol Chem. 1985 Dec 25;260(30):16089-98 PMID: 3905805
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.59.1.131-136.1991
Published
1991-01-00
Pages
131-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC257716
Subset
IM
Grants
NIGMS NIH HHS · GM-36054 · United States
External Links
PubMed source DOI: 10.1128/iai.59.1.131-136.1991 PMC Full Text View journal details
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