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PMID: 1898607 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The expression of the Ig H chain repertoire in developing bone marrow B lineage cells.

Journal of immunology (Baltimore, Md. : 1950) ·Vol. 146 ·No. 1 ·1991-01-01 ·Pages 350-61

Decker DJ, Boyle NE, Koziol JA, Klinman NR

Abstract

Analyses of rearranged Ig H chain V region genes of bone marrow pre-B cells demonstrate extensive sequence diversity, particularly within the third hypervariable region (HCDR3). This diversity is constrained, however, through preferential utilization of certain D gene segments and possibly VH gene segments and a preponderance of productive rearrangements, primarily those expressing D gene segments in a preferred reading frame. The predominance of productively rearranged V genes with D regions translated in a preferred frame, is, at least in part, the consequence of selective clonal expansion encompassing at least five to six divisions subsequent to VH-D-JH rearrangement. Selection for clonal expansion appears to be dependent on recognition of the nascent H chain product of certain productively rearranged genes.

MeSH Terms

Antibody Diversity B-Lymphocytes/cytology,physiology Base Sequence Bone Marrow Cells Cell Differentiation Clone Cells Gene Rearrangement, B-Lymphocyte, Heavy Chain Genes, Immunoglobulin Hematopoiesis Humans Immunoglobulin Heavy Chains/genetics Immunoglobulin Variable Region/genetics Molecular Sequence Data Polymerase Chain Reaction

Chemicals

Immunoglobulin Heavy Chains Immunoglobulin Variable Region

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Decker D J

Department of Immunology, Scripps Clinic & Research Foundation, La Jolla, CA 92037.

Boyle N E

Koziol J A

Klinman N R

Article Info

Journal

Journal of immunology (Baltimore, Md. : 1950)

Abbr.

J Immunol

ISSN

0022-1767

Published

1991-01-01

Pages

350-61

Language

English

Region

United States

NLM ID

2985117R

Subset

Grants

NIA NIH HHS · AG 01743 · United States

NIAID NIH HHS · AI 15797 · United States

Databases

GENBANK

M55703, M55704, M55705, M55706, M55707, M55708, M55709, M55710, M55711, M55712, M55713, M55714, M55715, M55716, M55717, M55718, M55719, M55720, M55721, M55722, M55723, M55724, M55725, M55726, M55727, M55728, M55729, M55730, M55731, M55732

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The expression of the Ig H chain repertoire in developing bone marrow B lineage cells.

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