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PMID: 18971212 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Review

Role of N-glycosylation in trafficking of apical membrane proteins in epithelia.

American journal of physiology. Renal physiology ·Vol. 296 ·No. 3 ·2009-03-00 ·Pages F459-69

Vagin O, Kraut JA, Sachs G

Abstract

Polarized distribution of plasma membrane transporters and receptors in epithelia is essential for vectorial functions of epithelia. This polarity is maintained by sorting of membrane proteins into apical or basolateral transport containers in the trans-Golgi network and/or endosomes followed by their delivery to the appropriate plasma membrane domains. Sorting depends on the recognition of sorting signals in proteins by specific sorting machinery. In the present review, we summarize experimental evidence for and against the hypothesis that N-glycans attached to the membrane proteins can act as apical sorting signals. Furthermore, we discuss the roles of N-glycans in the apical sorting event per se and their contribution to folding and quality control of glycoproteins in the endoplasmic reticulum or retention of glycoproteins in the plasma membrane. Finally, we review existing hypotheses on the mechanism of apical sorting and discuss the potential roles of the lectins, VIP36 and galectin-3, as putative apical sorting receptors.

MeSH Terms
Endoplasmic Reticulum/metabolism Epithelial Cells/metabolism Glycoproteins/metabolism Glycosylation Golgi Apparatus/metabolism Membrane Microdomains/metabolism Membrane Proteins/metabolism Protein Folding Protein Sorting Signals Protein Stability
Chemicals
Glycoproteins Membrane Proteins Protein Sorting Signals
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vagin Olga
Department of Physiology, David Geffen School of Medicine at University of California, Bldg. 113, Rm. 324, 11301 Wilshire Blvd., Los Angeles, California 90073, USA. olgav@ucla.edu
Kraut Jeffrey A
Sachs George
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Article Info
Journal
American journal of physiology. Renal physiology
Abbr.
Am J Physiol Renal Physiol
ISSN
1931-857X
DOI
10.1152/ajprenal.90340.2008
Email
olgav@ucla.edu
Published
2009-03-00
Epub
2008-00-29
Pages
F459-69
Language
English
Region
United States
NLM ID
100901990
PMCID
PMC2660186
Subset
IM
Grants
NIDDK NIH HHS · R01 DK077149-02 · United States
NIDDK NIH HHS · R01 DK077149 · United States
NIDDK NIH HHS · R01 DK077149-03 · United States
NIDDK NIH HHS · DK-053642 · United States
NIDDK NIH HHS · DK-58333 · United States
NIDDK NIH HHS · DK-077149 · United States
External Links
PubMed source DOI: 10.1152/ajprenal.90340.2008 PMC Full Text View journal details
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