Home LiteratureArticle Details
PMID: 1896445 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Assembly of combinatorial antibody libraries on phage surfaces: the gene III site.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 18 ·1991-09-15 ·Pages 7978-82

Barbas CF, Kang AS, Lerner RA, Benkovic SJ

Abstract

A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carboxyl-terminal domain of the gene III protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 10(3)- to 10(5)-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.

Related Genes
gene III
MeSH Terms
Antibodies, Bacterial/genetics Antibody Diversity Base Sequence Binding Sites, Antibody Capsid/genetics Cloning, Molecular Gene Expression Gene Library Genetic Vectors Immunoglobulin Fab Fragments/genetics Molecular Sequence Data Oligonucleotides/chemistry Recombinant Fusion Proteins Tetanus Toxoid/immunology
Chemicals
Antibodies, Bacterial Immunoglobulin Fab Fragments Oligonucleotides Recombinant Fusion Proteins Tetanus Toxoid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Barbas C F
Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.
Kang A S
Lerner R A
Benkovic S J
References (23)
23 references, click to expand
  1. In vitro selection of RNA molecules that bind specific ligands.
    Nature. 1990 Aug 30;346(6287):818-22 PMID: 1697402
  2. Searching for peptide ligands with an epitope library.
    Science. 1990 Jul 27;249(4967):386-90 PMID: 1696028
  3. Identification of clones that encode chicken tropomyosin by direct immunological screening of a cDNA expression library.
    Proc Natl Acad Sci U S A. 1983 Jan;80(1):31-5 PMID: 6185958
  4. Gene-III protein of filamentous phages: evidence for a carboxyl-terminal domain with a role in morphogenesis.
    Virology. 1984 Jan 30;132(2):445-55 PMID: 6608175
  5. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  6. Effects of bacteriophage f1 gene III protein on the host cell membrane.
    Mol Gen Genet. 1982;186(2):185-92 PMID: 6955583
  7. Hormone phage: an enrichment method for variant proteins with altered binding properties.
    Proteins. 1990;8(4):309-14 PMID: 1708882
  8. Human and mouse monoclonal antibodies by repertoire cloning.
    Trends Biotechnol. 1991 May;9(5):169-75 PMID: 1367284
  9. Membrane biogenesis: cotranslational integration of the bacteriophage f1 coat protein into an Escherichia coli membrane fraction.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1251-5 PMID: 375232
  10. Generation of diverse high-affinity human monoclonal antibodies by repertoire cloning.
    Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2432-6 PMID: 1826052
  11. Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces.
    Proc Natl Acad Sci U S A. 1991 May 15;88(10):4363-6 PMID: 1903540
  12. At the crossroads of chemistry and immunology: catalytic antibodies.
    Science. 1991 May 3;252(5006):659-67 PMID: 2024118
  13. Phage antibodies: filamentous phage displaying antibody variable domains.
    Nature. 1990 Dec 6;348(6301):552-4 PMID: 2247164
  14. Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.
    Gene. 1989 Apr 15;77(1):61-8 PMID: 2744488
  15. Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties.
    Nucleic Acids Res. 1988 Aug 11;16(15):7583-600 PMID: 2970625
  16. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  17. Antibody-selectable filamentous fd phage vectors: affinity purification of target genes.
    Gene. 1988 Dec 20;73(2):305-18 PMID: 3149606
  18. Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.
    Proc Natl Acad Sci U S A. 1988 Aug;85(16):5879-83 PMID: 3045807
  19. Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda.
    Science. 1989 Dec 8;246(4935):1275-81 PMID: 2531466
  20. Cloning of the immunological repertoire in Escherichia coli for generation of monoclonal catalytic antibodies: construction of a heavy chain variable region-specific cDNA library.
    Proc Natl Acad Sci U S A. 1989 Aug;86(15):5728-32 PMID: 2503822
  21. Random peptide libraries: a source of specific protein binding molecules.
    Science. 1990 Jul 27;249(4967):404-6 PMID: 2143033
  22. Influenza virus hemagglutinin-specific antibodies isolated from a combinatorial expression library are closely related to the immune response of the donor.
    Proc Natl Acad Sci U S A. 1990 Aug;87(16):6450-4 PMID: 1696733
  23. Peptides on phage: a vast library of peptides for identifying ligands.
    Proc Natl Acad Sci U S A. 1990 Aug;87(16):6378-82 PMID: 2201029
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.18.7978
Published
1991-09-15
Pages
7978-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52428
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.18.7978 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2