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PMID: 1896007 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Characterization and sequence of a novel nitrate reductase from barley.

Molecular & general genetics : MGG ·Vol. 228 ·No. 3 ·1991-09-00 ·Pages 329-34

Miyazaki J, Juricek M, Angelis K, Schnorr KM, Kleinhofs A, Warner RL

Abstract

Barley (Hordeum vulgare L.) has both NADH-specific and NAD(P)H-bispecific nitrate reductases. Genomic and cDNA clones of the NADH nitrate reductase have been sequenced. In this study, a genomic clone (pMJ4.1) of a second type of nitrate reductase was isolated from barley by homology to a partial-length NADH nitrate reductase cDNA and the sequence determined. The open reading frame encodes a polypeptide of 891 amino acids and its interrupted by two small introns. The deduced amino acid sequence has 70% identity to the barley NADH-specific nitrate reductase. The non-coding regions of the pMJ4.1 gene have low homology (ca. 40%) to the corresponding regions of the NADH nitrate reductase gene. Expression of the pMJ4.1 nitrate reductase gene is induced by nitrate in root tissues which corresponds to the induction of NAD(P)H nitrate reductase activity. The pMJ4.1 nitrate reductase gene is sufficiently different from all previously reported higher plant nitrate reductase genes to suggest that it encodes the barley NAD(P)H-bispecific nitrate reductase.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern DNA/genetics Hordeum/enzymology Molecular Sequence Data Nitrate Reductase Nitrate Reductase (NAD(P)H) Nitrate Reductase (NADH) Nitrate Reductases/genetics RNA, Messenger/genetics Sequence Homology, Nucleic Acid
Chemicals
RNA, Messenger DNA Nitrate Reductases Nitrate Reductase (NADH) Nitrate Reductase (NAD(P)H) Nitrate Reductase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Miyazaki J
Department of Crop and Soil Sciences, Washington State University, Pullman 99164-6420.
Juricek M
Angelis K
Schnorr K M
Kleinhofs A
Warner R L
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00260624
Published
1991-09-00
Pages
329-34
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
S47117, S55122, X60173, X63981, X63982, X63983, X63984, X63985, X63986, X63987
External Links
PubMed source DOI: 10.1007/BF00260624
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