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PMID: 1895404 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcriptional and posttranscriptional regulation of class I major histocompatibility complex genes following transformation with human adenoviruses.

Journal of virology ·Vol. 65 ·No. 10 ·1991-10-00 ·Pages 5544-8

Shemesh J, Rotem-Yehudar R, Ehrlich R

Abstract

Transformation of rodent cells by human adenoviruses is a well-established model system for studying the expression, regulation, and function of class I antigens. In this report, we demonstrate that the highly oncogenic adenovirus type 12 operates at the transcriptional and posttranscriptional levels in regulating the activity of major histocompatibility complex class I genes and products in transformed cells. Adenovirus type 12 suppresses the cell surface expression of class I antigens in most cell lines. Nevertheless, in a number of cell lines suppression is the result of reduction in the amount of stable specific mRNA, while in another group of cell lines suppression involves interference with processing of a posttranscriptional product. The two mechanisms operate both for the endogenous H-2 genes and for a miniature swine class I transgene that is expressed in the cells.

MeSH Terms

Adenoviruses, Human/genetics,immunology Animals Cell Line Gene Expression Regulation, Viral Genes, MHC Class I H-2 Antigens/analysis,genetics Mice Mice, Transgenic Suppression, Genetic Swine Swine, Miniature Transcription, Genetic

Chemicals

H-2 Antigens

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Shemesh J

Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.

Rotem-Yehudar R

Ehrlich R

References (33)

33 references, click to expand

Characterization of a porcine genomic clone encoding a major histocompatibility antigen: expression in mouse L cells.
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1403-7 PMID: 6461859
A cDNA clone containing the entire coding sequence of a mouse H-2Kd histocompatibility antigen.
Nucleic Acids Res. 1983 Mar 11;11(5):1567-77 PMID: 6298749
Monoclonal antibody to an alloantigenic determinant on beta2-microglobulin (beta 2M) of the mouse.
Immunogenetics. 1982;16(1):91-3 PMID: 6180984
Spectrum of tumorigenic phenotypes among adenovirus 2-, adenovirus 12-, and simian virus 40-transformed Syrian hamster cells defined by host cellular immune-tumor cell interactions.
Cancer Res. 1982 Mar;42(3):939-44 PMID: 6277479
Expression of class I major histocompatibility antigens switched off by highly oncogenic adenovirus 12 in transformed rat cells.
Nature. 1983 Oct 27-Nov 2;305(5937):771-5 PMID: 6355856
Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression.
J Immunol. 1981 Jan;126(1):317-21 PMID: 6935293
CTL recognition of adenovirus-transformed cells infected with influenza virus: lysis by anti-influenza CTL parallels adenovirus-12-induced suppression of class I MHC molecules.
Virology. 1988 Jan;162(1):236-8 PMID: 2827381
Identification of negative and positive regulatory elements associated with a class I major histocompatibility complex gene.
Mol Cell Biol. 1988 Feb;8(2):695-703 PMID: 2832733
Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes.
Cell. 1980 May;20(1):95-105 PMID: 6893015
Adenovirus type 12 early region 1A proteins repress class I HLA expression in transformed human cells.
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5257-61 PMID: 3014539
Adenovirus type 12 E1A gene represses accumulation of MHC class I mRNAs at the level of transcription.
Virology. 1988 Jul;165(1):303-5 PMID: 2968712
MHC-restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction-specificity, function, and responsiveness.
Adv Immunol. 1979;27:51-177 PMID: 92183
Host response to adenovirus 2-transformed hamster embryo cells.
Cancer Res. 1979 May;39(5):1455-61 PMID: 427789
Human adenoviruses: growth, purification, and transfection assay.
Methods Enzymol. 1979;58:425-35 PMID: 423779
Increased MHC H-2K gene transcription in cultured mouse embryo cells after adenovirus infection.
Nature. 1985 Jun 13-19;315(6020):579-81 PMID: 2989693
Histocompatibility antigens on murine tumors.
Science. 1985 Nov 15;230(4727):777-83 PMID: 2997918
Impaired intracellular transport of class I MHC antigens as a possible means for adenoviruses to evade immune surveillance.
Cell. 1985 Nov;43(1):215-22 PMID: 2934137
"E3/19K" protein of adenovirus type 2 inhibits lysis of cytolytic T lymphocytes by blocking cell-surface expression of histocompatibility class I antigens.
Proc Natl Acad Sci U S A. 1987 Mar;84(5):1356-60 PMID: 2950523
The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species.
Vet Immunol Immunopathol. 1987 Jul;15(4):337-76 PMID: 3114940
Regulation of major histocompatibility class I gene expression at the level of transcription in highly oncogenic adenovirus transformed rat cells.
Oncogene. 1988 Oct;3(4):483-7 PMID: 2484015
Expression of a class I MHC transgene: effects of in vivo alpha/beta-interferon treatment.
Immunogenetics. 1989;30(1):18-26 PMID: 2473029
Isolation of cytoplasmic RNA: ribonucleoside-vanadyl complexes.
Methods Enzymol. 1987;152:227-34 PMID: 2443795
Adenovirus type 12 E1A down regulates expression of a transgene under control of a major histocompatibility complex class I promoter: evidence for transcriptional control.
J Virol. 1989 Sep;63(9):4039-42 PMID: 2527313
Tumorigenicity of adenovirus-transformed rat cells and expression of class I major histocompatibility antigen.
Virology. 1984 Apr 30;134(2):460-5 PMID: 6242485
The influence of major histocompatibility complex class I antigens on tumor growth and metastasis.
Biochim Biophys Acta. 1987 Nov 25;907(3):245-59 PMID: 3314998
Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db.
Proc Natl Acad Sci U S A. 1986 Oct;83(19):7447-51 PMID: 3532114
Tumorigenicity of hamster and mouse cells transformed by adenovirus types 2 and 5 is not influenced by the level of class I major histocompatibility antigens expressed on the cells.
Proc Natl Acad Sci U S A. 1986 Dec;83(24):9684-8 PMID: 3467332
Post-transcriptional control of class I MHC mRNA expression in adenovirus 12-transformed cells.
Science. 1987 Mar 20;235(4795):1486-8 PMID: 3823900
Expression of histocompatibility antigens H-2K, -D, and -L is reduced in adenovirus-12-transformed mouse cells and is restored by interferon gamma.
Proc Natl Acad Sci U S A. 1985 Aug;82(16):5525-9 PMID: 3927302
Expression of a microinjected porcine class I major histocompatibility complex gene in transgenic mice.
Science. 1985 May 3;228(4699):577-80 PMID: 3885396
Reversal of oncogenesis by the expression of a major histocompatibility complex class I gene.
Science. 1985 Apr 5;228(4695):26-30 PMID: 3975631
Identification of sequences responsible for positive and negative regulation by E1A in the promoter of H-2Kbm1 class I MHC gene.
EMBO J. 1990 Jan;9(1):127-35 PMID: 2136829
Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition sites.
Nature. 1984 May 17-23;309(5965):279-81 PMID: 6201750

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.65.10.5544-5548.1991

Published

1991-10-00

Pages

5544-8

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC249056

Subset

External Links

PubMed source DOI: 10.1128/JVI.65.10.5544-5548.1991 PMC Full Text View journal details

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Transcriptional and posttranscriptional regulation of class I major histocompatibility complex genes following transformation with human adenoviruses.

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