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PMID: 18937087 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

E-cadherin/catenin complexes are formed cotranslationally in the endoplasmic reticulum/Golgi compartments.

Cell communication & adhesion ·Vol. 15 ·No. 4 ·2008-11-00 ·Pages 365-78

Curtis MW, Johnson KR, Wheelock MJ

Abstract

Cadherins are synthesized with a proregion that lies between a short amino-terminal signal sequence and the first extracellular domain. Following synthesis, the proregion is cleaved, an event that is mandatory for the mature cadherin to function in adhesion. The authors have previously reported that catenins coimmunoprecipate with pro-N-cadherin, and that the N-cadherin/catenin complex forms in the Golgi/endoplasmic reticulum. It is clear that N- and E-cadherin confer significantly different characteristics on cells, and it is possible that N- and E-cadherin/catenin complex formation is equally different. To investigate this, the authors generated an antibody against the proregion of E-cadherin and have used it to examine the assembly of the E-cadherin/catenin complex.

MeSH Terms
Biological Transport Cadherins/biosynthesis,metabolism Catenins/biosynthesis,metabolism Cell Adhesion Molecules/metabolism Cell Membrane/metabolism Cells, Cultured Cytoskeleton/metabolism Endoplasmic Reticulum/metabolism Golgi Apparatus/metabolism Humans Immunoprecipitation Phosphoproteins/metabolism Protein Binding Protein Biosynthesis
Chemicals
Cadherins Catenins Cell Adhesion Molecules Phosphoproteins delta catenin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Curtis Matthew W
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska 68198-7696, USA.
Johnson Keith R
Wheelock Margaret J
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Article Info
Journal
Cell communication & adhesion
Abbr.
Cell Commun Adhes
ISSN
1543-5180
DOI
10.1080/15419060802460748
Published
2008-11-00
Pages
365-78
Language
English
Region
England
NLM ID
101096596
PMCID
PMC2742162
Subset
IM
Grants
NIGMS NIH HHS · R01-GM51188 · United States
NIDCR NIH HHS · R01-DE12308 · United States
NIDCR NIH HHS · R01 DE012308 · United States
NCI NIH HHS · P30 CA036727-22 · United States
NIGMS NIH HHS · R01 GM051188-08 · United States
NCI NIH HHS · P30 CA036727 · United States
NIDCR NIH HHS · R01 DE012308-08 · United States
NCI NIH HHS · P30 CA36727 · United States
NIGMS NIH HHS · R01 GM051188 · United States
External Links
PubMed source DOI: 10.1080/15419060802460748 PMC Full Text
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