Enzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 1 ·1977-01-00 ·Pages 96-100

Gillam S, Rottman F, Jahnke P, Smith M

Abstract

The deoxyribooligonucleotide, d(pT-T-A-G-C-A-G-A-A-C-C-G-G), constituting a segment of yeast iso-1-cytochrome c gene, has been synthesized by a combination of chemical and primarily enzymatic methods. The starting primer, d(pT-T-A-G1, was chemically synthesized by the phosphodiester method and was extended stepwise, by reactions catalyzed by polynucleotide phosphorylase.

MeSH Terms

Cytochrome c Group Genes Oligodeoxyribonucleotides/analysis,chemical synthesis Oligonucleotides/chemical synthesis Polyribonucleotide Nucleotidyltransferase/metabolism Saccharomyces cerevisiae

Chemicals

Cytochrome c Group Oligodeoxyribonucleotides Oligonucleotides Polyribonucleotide Nucleotidyltransferase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Gillam S

Rottman F

Jahnke P

Smith M

References (11)

11 references, click to expand

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Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence.
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Catalysis of DNA joining by bacteriophage T4 RNA ligase.
Biochem Biophys Res Commun. 1976 Jan 26;68(2):417-24 PMID: 766755
THE EFFECT OF UREA, FORMAMIDE, AND GLYCOLS ON THE SECONDARY BINDING FORCES IN THE ION-EXCHANGE CHROMATOGRAPHY OF POLYNUCLEOTIDES OF DEAE-CELLULOSE.
Biochemistry. 1963 Jul-Aug;2:697-702 PMID: 14075100
Enzymatic synthesis of oligonucleotides of defined sequence. Addition of short blocks of nucleotide residues to oligonucleotide primers.
Nucleic Acids Res. 1975 May;2(5):613-24 PMID: 167349
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.74.1.96

Published

1977-01-00

Pages

96-100

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC393204

Subset

External Links

PubMed source DOI: 10.1073/pnas.74.1.96 PMC Full Text View journal details

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Enzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene.

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