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PMID: 1893098 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evidence for an association of the early light-inducible protein (ELIP) of pea with photosystem II.

Plant molecular biology ·Vol. 16 ·No. 2 ·1991-02-00 ·Pages 209-23

Adamska I, Kloppstech K

Abstract

The precursor to the nuclear-coded 17 kDa early light-inducible protein (ELIP) of pea has been transported into isolated intact chloroplasts. The location of the mature protein in the thylakoid membranes was investigated after using cleavable crosslinkers such as DSP and SAND in conjunction with immunofractionation methods and by application of mild detergent fractionation. We show that ELIP is integrated into the membranes via the unstacked stroma thylakoids. After isolation of protein complexes by solubilization of membranes with Triton X-100 and sucrose density-gradient centrifugation the crosslinked ELIP comigrates with the PS II core complex. Using SAND we identified ELIP as a 41-51 kDa crosslinked product while with DSP four products of 80 kDa, 70 kDa, 50-42 kDa and 23-21 kDa were found. The immunoprecipitation data suggested that the D1-protein of the PS II complex is one of the ELIP partners in crosslinked products.

MeSH Terms
Azides Centrifugation, Density Gradient Chloroplasts/metabolism Cross-Linking Reagents Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Fabaceae/genetics,metabolism Membrane Proteins/metabolism Photosynthetic Reaction Center Complex Proteins/metabolism Photosystem II Protein Complex Plant Proteins/metabolism Plants, Medicinal Precipitin Tests Succinimides
Chemicals
Azides Cross-Linking Reagents Membrane Proteins Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Proteins Succinimides sulfosuccinimidyl-2-(3-azido-2-nitrobenzamido)ethyl-1,3'-dithiopropionate dithiobis(succinimidylpropionate)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Adamska I
Institut für Botanik, Universität Hannover, Germany.
Kloppstech K
References (41)
41 references, click to expand
  1. Optimal conditions for post-translational uptake of proteins by isolated chloroplasts. In vitro synthesis and transport of plastocyanin, ferredoxin-NADP+ oxidoreductase, and fructose-1,6-bisphosphatase.
    J Biol Chem. 1982 Feb 10;257(3):1558-63 PMID: 6276392
  2. Chloroplasts.
    J Cell Biol. 1981 Dec;91(3 Pt 2):256s-270s PMID: 6172430
  3. An improved method for isolation of H-2 and Ia alloantigens with immunoprecipitation induced by protein A-bearing staphylococci.
    J Immunol. 1976 Jul;117(1):136-42 PMID: 58931
  4. Topology and neighbor analysis of the photosynthetic reaction center from Rhodopseudomonas sphaeroides.
    J Biol Chem. 1985 Jun 10;260(11):6932-7 PMID: 3888987
  5. Light-stimulated transcription of genes for two chloroplast polypeptides in isolated pea leaf nuclei.
    EMBO J. 1982;1(12):1493-8 PMID: 16453439
  6. Organ-specific and light-induced expression of plant genes.
    Science. 1986 May 30;232(4754):1106-12 PMID: 17754498
  7. Control of expression of chloroplast membrane proteins in higher plants.
    Prog Clin Biol Res. 1982;102 Pt B:101-10 PMID: 7163164
  8. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  9. Transport of proteins into chloroplasts. Binding of nuclear-coded chloroplast proteins to the chloroplast envelope.
    Eur J Biochem. 1982 Aug;126(1):143-8 PMID: 6181990
  10. Identification of neighboring protein pairs in rat liver 60S ribosomal subunits cross-linked with dimethyl suberimidate or dimethyl 3,3'-dithiobispropionimidate.
    J Biochem. 1980 Oct;88(4):1033-44 PMID: 7451403
  11. The application of chemical crosslinking for studies on cell membranes and the identification of surface reporters.
    Biochim Biophys Acta. 1979 Apr 23;559(1):39-69 PMID: 156043
  12. Increased sensitivity in peroxidase immunocytochemistry. A comparative study of a number of peroxidase visualization methods employing a model system.
    Histochemistry. 1986;84(3):221-30 PMID: 3519544
  13. An approach to nearest neighbor analysis of membrane proteins. Application to the human erythrocyte membrane of a method employing cleavable cross-linkages.
    J Biol Chem. 1974 Dec 25;249(24):8005-18 PMID: 4430682
  14. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.
    Anal Biochem. 1980 Jul 1;105(2):361-3 PMID: 6161559
  15. Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4927-31 PMID: 291909
  16. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  17. Photoactivated heterobifunctional cross-linking reagents which demonstrate the aggregation state of phospholipase A2.
    Biochemistry. 1977 Dec 13;16(25):5650-4 PMID: 921957
  18. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.
    Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330-4 PMID: 4525168
  19. Intramembrane translocation and posttranslational palmitoylation of the chloroplast 32-kDa herbicide-binding protein.
    Proc Natl Acad Sci U S A. 1987 Mar;84(6):1497-501 PMID: 3550792
  20. Organization of the photosynthetic units, and onset of electron transport and excitation energy distribution in greening leaves.
    Photosynth Res. 1986 Jan;10(3):171-80 PMID: 24435362
  21. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  22. Regulation of protein metabolism: Coupling of photosynthetic electron transport to in vivo degradation of the rapidly metabolized 32-kilodalton protein of the chloroplast membranes.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1380-4 PMID: 16593427
  23. Functional and mutational analysis of the light-harvesting chlorophyll a/b protein of thylakoid membranes.
    J Cell Biol. 1986 Mar;102(3):972-81 PMID: 3512583
  24. Differential expression of the genes for ribulose-1,5-bisphosphate carboxylase and light-harvesting chlorophyll a/b protein in the developing barley leaf.
    Planta. 1980 Nov;150(1):41-5 PMID: 24306524
  25. Isolation of messenger RNA from polysomes by chromatography on oligo(dT)-cellulose.
    Anal Biochem. 1975 May 26;66(1):18-28 PMID: 1170770
  26. Expression of genes for plastid membrane proteins in barley under intermittent light conditions.
    Planta. 1982 Mar;154(1):18-23 PMID: 24275912
  27. Investigation of the spatial relationships between photosystem 2 polypeptides by reversible crosslinking and diagonal electrophoresis.
    Photosynth Res. 1987 Jan;13(2):185-98 PMID: 24435786
  28. A rapidly light-induced chloroplast protein with a high turnover coded for by pea nuclear DNA.
    Eur J Biochem. 1984 Jan 2;138(1):201-7 PMID: 6692824
  29. The plastid membranes of barley (Hordeum vulgare). Light-induced appearance of mRNA coding for the apoprotein of the light-harvesting chlorophyll a/b protein.
    Eur J Biochem. 1978 Apr 17;85(2):581-8 PMID: 648535
  30. Characterization of chloroplast photosystems 1 and 2 separated by a non-detergent method.
    Biochim Biophys Acta. 1970 Aug 4;216(1):162-78 PMID: 5497183
  31. Detection of antigens on nitrocellulose paper immunoblots with monoclonal antibodies.
    Anal Biochem. 1983 Aug;133(1):214-9 PMID: 6356979
  32. Diurnal and circadian rhythmicity in the expression of light-induced plant nuclear messenger RNAs.
    Planta. 1985 Sep;165(4):502-6 PMID: 24241223
  33. Control of psbA gene expression: in mature Spirodela chloroplasts light regulation of 32-kd protein synthesis is independent of transcript level.
    EMBO J. 1985 Feb;4(2):291-5 PMID: 16453605
  34. Biosynthesis of the light-harvesting chlorophyll a/b protein. Control of messenger RNA activity by light.
    Eur J Biochem. 1981 Aug;118(1):71-80 PMID: 6169525
  35. The early light-inducible proteins of barley. Characterization of two families of 2-h-specific nuclear-coded chloroplast proteins.
    Eur J Biochem. 1987 Sep 15;167(3):493-9 PMID: 2443350
  36. The rapidly metabolized 32,000-dalton polypeptide of the chloroplast is the "proteinaceous shield" regulating photosystem II electron transport and mediating diuron herbicide sensitivity.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1572-6 PMID: 6940173
  37. Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system.
    J Biol Chem. 1971 Oct 25;246(20):6328-34 PMID: 5127429
  38. Circadian Rhythm in the Expression of the mRNA Coding for the Apoprotein of the Light-Harvesting Complex of Photosystem II : Phytochrome Control and Persistent Far Red Reversibility.
    Plant Physiol. 1989 Jun;90(2):665-72 PMID: 16666825
  39. Transiently expressed early light-inducible thylakoid proteins share transmembrane domains with light-harvesting chlorophyll binding proteins.
    Plant Mol Biol. 1989 Nov;13(5):583-93 PMID: 2491675
  40. Circadian Control of the Accumulation of mRNAs for Light- and Heat-Inducible Chloroplast Proteins in Pea (Pisum sativum L.).
    Plant Physiol. 1988 Sep;88(1):21-5 PMID: 16666268
  41. A new radioactive cross-linking reagent for studying the interactions of proteins.
    J Biol Chem. 1982 Mar 10;257(5):2343-9 PMID: 7061425
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00020553
Published
1991-02-00
Pages
209-23
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00020553 View journal details
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