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PMID: 18927436 Published · ppublish English Journal Article

Reductive isolation from bone marrow and blood implicates common lymphoid progenitors as the major source of thymopoiesis.

Blood ·Vol. 113 ·No. 4 ·2009-01-22 ·Pages 807-15

Serwold T, Ehrlich LI, Weissman IL

Abstract

Ongoing thymopoiesis requires continual seeding from progenitors that reside within the bone marrow (BM), but the identity of the most proximate prethymocytes has remained controversial. Here we take a comprehensive approach to prospectively identify the major source of thymocyte progenitors that reside within the BM and blood, and find that all thymocyte progenitor activity resides within a rare Flk2(+)CD27(+) population. The BM Flk2(+)CD27(+) subset is predominantly composed of common lymphoid progenitors (CLPs) and multipotent progenitors. Of these 2 populations, only CLPs reconstitute thymopoiesis rapidly after intravenous injection. In contrast, multipotent progenitor-derived cells reconstitute the thymus with delayed kinetics only after they have reseeded the BM, self-renewed, and generated CLPs. These results identify CLPs as the major source of thymocyte progenitors within the BM.

MeSH Terms
Animals Blood Cells/cytology,immunology Bone Marrow Cells/cytology,immunology Cell Lineage/immunology Cell Separation/methods Cells, Cultured Hematopoiesis/immunology Kinetics Lymphoid Progenitor Cells/cytology,immunology Membrane Proteins/immunology Mice Mice, Inbred C57BL Spleen/cytology,immunology Thymus Gland/cytology,immunology Time Factors Tumor Necrosis Factor Receptor Superfamily, Member 7/immunology
Chemicals
Membrane Proteins Tumor Necrosis Factor Receptor Superfamily, Member 7 flt3 ligand protein
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Serwold Thomas
Stanford Institute of Stem Cell Biology and Regenerative Medicine, CA, USA.
Ehrlich L I R
Weissman Irving L
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Article Info
Journal
Blood
Abbr.
Blood
ISSN
1528-0020
DOI
10.1182/blood-2008-08-173682
Published
2009-01-22
Epub
2008-00-16
Pages
807-15
Language
English
Region
United States
NLM ID
7603509
PMCID
PMC4123410
Subset
IM
External Links
PubMed source DOI: 10.1182/blood-2008-08-173682 PMC Full Text View journal details
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