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PMID: 18849468 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

H3K9 methylation regulates growth and development in Aspergillus fumigatus.

Eukaryotic cell ·Vol. 7 ·No. 12 ·2008-12-00 ·Pages 2052-60

Palmer JM, Perrin RM, Dagenais TR, Keller NP

Abstract

In most species, chromatin remodeling mediates critical biological processes ranging from development to disease states. In fungi within the genus Aspergillus, chromatin remodeling may regulate expression of metabolic gene clusters, but other processes regulated by chromatin structure remain to be elucidated. In many eukaryotic species, methylation of lysine 9 of histone 3 (H3K9) is a hallmark of heterochromatin formation and subsequent gene silencing. The sole H3K9 methyltransferase in Schizosaccharomyces pombe is Clr4. We report that disruption of the Clr4 homolog in the pathogenic mold Aspergillus fumigatus (ClrD), which is involved in both mono- and trimethylation of H3K9, results in several growth abnormalities. Developmental defects in DeltaAfclrD include reduction in radial growth, reduction in conidial production, and delayed conidiation after developmental competence mediated by delayed expression of brlA, the master regulator of conidiophore development. Sensitivity of DeltaAfclrD to 6-azauracil suggests that ClrD influences transcriptional processing in A. fumigatus. Despite growth abnormalities, macrophage assays suggest ClrD may be dispensable for host interactions.

MeSH Terms
Animals Aspergillus fumigatus/enzymology,genetics,growth & development,metabolism Cell Line Fungal Proteins/genetics,metabolism Gene Expression Regulation, Fungal Histone-Lysine N-Methyltransferase/genetics,metabolism Histones/genetics,metabolism Lysine/genetics,metabolism Macrophages/microbiology Methylation Mice Protein Processing, Post-Translational
Chemicals
Fungal Proteins Histones Histone-Lysine N-Methyltransferase Lysine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Palmer Jonathan M
Department of Plant Pathology, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Perrin Robyn M
Dagenais Taylor R T
Keller Nancy P
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Article Info
Journal
Eukaryotic cell
Abbr.
Eukaryot Cell
ISSN
1535-9786
DOI
10.1128/EC.00224-08
Published
2008-12-00
Epub
2008-00-10
Pages
2052-60
Language
English
Region
United States
NLM ID
101130731
PMCID
PMC2593193
Subset
IM
Grants
NIAID NIH HHS · R01 AI065728 · United States
NIAID NIH HHS · R01 AI065728-01A1 · United States
External Links
PubMed source DOI: 10.1128/EC.00224-08 PMC Full Text
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