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PMID: 1883875 Published · ppublish English Journal Article

Localization of transforming growth factor-beta 1 in mitochondria of murine heart and liver.

Cell regulation ·Vol. 2 ·No. 6 ·1991-06-00 ·Pages 467-77

Heine UI, Burmester JK, Flanders KC, Danielpour D, Munoz EF, Roberts AB, Sporn MB

Abstract

Using both electron microscopic immunohistochemistry and cell fractionation techniques, we show that transforming growth factor-beta 1 (TGF-beta 1) is found in mitochondria of rat and mouse cardiac myocytes and rat hepatocytes. Four different polyclonal antibodies, raised against various epitopes encompassing the mature portion of the TGF-beta 1 molecule as well as the pro-region of its precursor, were used for the electron microscopy studies. The localization of TGF-beta 1 in mitochondria was confirmed by detection of the native peptide in mitochondria isolated from rat heart and liver; the majority of native TGF-beta 1 found in liver homogenates was recovered in highly pure mitochondrial fractions. The functional role of TGF-beta in the mitochondrion is unknown at present.

MeSH Terms
Animals Cell Fractionation Immunohistochemistry Mice Mitochondria, Heart/chemistry Mitochondria, Liver/chemistry Rats Rats, Inbred Strains Transforming Growth Factor beta/analysis
Chemicals
Transforming Growth Factor beta
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Heine U I
Laboratory of Chemoprevention, National Cancer Institute, Bethesda, Maryland 20892.
Burmester J K
Flanders K C
Danielpour D
Munoz E F
Roberts A B
Sporn M B
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Article Info
Journal
Cell regulation
Abbr.
Cell Regul
ISSN
1044-2030
DOI
10.1091/mbc.2.6.467
Published
1991-06-00
Pages
467-77
Language
English
Region
United States
NLM ID
9005331
PMCID
PMC361831
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.2.6.467 PMC Full Text
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