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PMID: 18832153 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cohesin subunit SMC1 associates with mitotic microtubules at the spindle pole.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 105 ·No. 40 ·2008-10-07 ·Pages 15441-5

Wong RW, Blobel G

Abstract

Accurate mitotic chromosome segregation depends on the formation of a microtubule-based bipolar spindle apparatus. We report that the cohesin subunit structural maintenance of chromosomes subunit 1 (SMC1) is recruited to microtubule-bound RNA export factor 1 (Rae1) at the mitotic spindle pole. We locate the Rae1-binding site to a 21-residue-long region, SMC1(947-967) and provide several lines of evidence that phosphorylation of Ser(957) and Ser(966) of SMC1 stimulates binding to Rae1. Imbalances in these assembly pathways caused formation of multipolar spindles. Our data suggest that cohesin's known bundling function for chromatids in mitotic and interphase cells extends to microtubules at the spindle pole.

MeSH Terms
Antigens, Nuclear/metabolism Ataxia Telangiectasia Mutated Proteins Binding Sites Cell Cycle Proteins/analysis,metabolism Chromosomal Proteins, Non-Histone/analysis,metabolism DNA-Binding Proteins/analysis,metabolism Fluorescent Antibody Technique HeLa Cells Humans Microtubules/metabolism Nuclear Matrix-Associated Proteins/analysis,metabolism Nucleocytoplasmic Transport Proteins/analysis,metabolism Phosphorylation Protein Serine-Threonine Kinases/analysis,metabolism Spindle Apparatus/metabolism,ultrastructure Transfection Tumor Suppressor Proteins/analysis,metabolism
Chemicals
Antigens, Nuclear Cell Cycle Proteins Chromosomal Proteins, Non-Histone DNA-Binding Proteins NUMA1 protein, human Nuclear Matrix-Associated Proteins Nucleocytoplasmic Transport Proteins RAE1 protein, human Tumor Suppressor Proteins structural maintenance of chromosome protein 1 ATM protein, human Ataxia Telangiectasia Mutated Proteins Protein Serine-Threonine Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wong Richard W
Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA. rwong@staff.kanazawa-u.ac.jp
Blobel Günter
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.0807660105
Email
rwong@staff.kanazawa-u.ac.jp
Published
2008-10-07
Epub
2008-00-01
Pages
15441-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2557025
Subset
IM
Grants
Howard Hughes Medical Institute · United States
External Links
PubMed source DOI: 10.1073/pnas.0807660105 PMC Full Text View journal details
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