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PMID: 1881889 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

RNA polymerase II-associated proteins are required for a DNA conformation change in the transcription initiation complex.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 17 ·1991-09-01 ·Pages 7509-13

Buratowski S, Sopta M, Greenblatt J, Sharp PA

Abstract

Proteins purified on the basis of their affinity for RNA polymerase II effectively substitute for previously defined transcription initiation factors. In two assays, formation of initiation complexes and transcription in vitro, the RNA polymerase II-associated proteins behaved identically to a fraction containing transcription factors IIE and IIF. Both fractions greatly stabilized the association of polymerase with the promoter and were required for the formation of complete initiation complexes. By using the DNA-cleaving reagent phenanthroline.copper in footprinting reactions, the RNA polymerase II-associated proteins were shown to be required for a DNA conformation change near the initiation site of the promoter. Based on similarity to the prokaryotic transcription complex, this conformation change is likely to represent a transition from a closed to an open complex.

MeSH Terms
Cellulose/analogs & derivatives Chromatography, Affinity DNA/chemistry,metabolism HeLa Cells/metabolism Humans Nucleic Acid Conformation Promoter Regions, Genetic RNA Polymerase II/isolation & purification,metabolism Recombinant Proteins/isolation & purification,metabolism Transcription Factor TFIIB Transcription Factor TFIID Transcription Factors/genetics,isolation & purification,metabolism Transcription Factors, TFII Transcription, Genetic
Chemicals
DNA-cellulose Recombinant Proteins Transcription Factor TFIIB Transcription Factor TFIID Transcription Factors Transcription Factors, TFII transcription factor TFIIE Cellulose DNA RNA Polymerase II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Buratowski S
Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
Sopta M
Greenblatt J
Sharp P A
References (50)
50 references, click to expand
  1. Factors involved in specific transcription in mammalian RNA polymerase II. Functional analysis of initiation factors IIA and IID and identification of a new factor operating at sequences downstream of the initiation site.
    J Biol Chem. 1987 Mar 5;262(7):3322-30 PMID: 3818643
  2. An RNA polymerase II transcription system from rat liver. Purification of an essential component.
    J Biol Chem. 1987 Jun 15;262(17):8293-7 PMID: 3597375
  3. Purification and characterization of a specific RNA polymerase II transcription factor.
    J Biol Chem. 1986 Feb 15;261(5):2003-13 PMID: 3944124
  4. Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4394-8 PMID: 3925456
  5. Isolation of three proteins that bind to mammalian RNA polymerase II.
    J Biol Chem. 1985 Aug 25;260(18):10353-60 PMID: 3860504
  6. Striking conservation of TFIID in Schizosaccharomyces pombe and Saccharomyces cerevisiae.
    Nature. 1990 Jul 19;346(6281):291-4 PMID: 2197558
  7. Functional domains and upstream activation properties of cloned human TATA binding protein.
    Science. 1990 Jun 29;248(4963):1625-30 PMID: 2363050
  8. Arabidopsis thaliana contains two genes for TFIID.
    Nature. 1990 Jul 26;346(6282):390-4 PMID: 2197561
  9. An RNA polymerase II transcription factor shares functional properties with Escherichia coli sigma 70.
    Science. 1990 Jun 22;248(4962):1550-3 PMID: 2193400
  10. Cloning of a transcriptionally active human TATA binding factor.
    Science. 1990 Jun 29;248(4963):1646-50 PMID: 2194289
  11. Factors involved in specific transcription by mammalian RNA polymerase II. Purification and subunit composition of transcription factor IIF.
    J Biol Chem. 1990 Apr 5;265(10):5629-34 PMID: 2180931
  12. Highly conserved core domain and unique N terminus with presumptive regulatory motifs in a human TATA factor (TFIID).
    Nature. 1990 Jul 26;346(6282):387-90 PMID: 2374612
  13. Sequencing and expression of complementary DNA for the general transcription factor BTF3.
    Nature. 1990 Apr 5;344(6266):556-9 PMID: 2320128
  14. Separation and characterization of factors mediating accurate transcription by RNA polymerase II.
    J Biol Chem. 1982 Dec 10;257(23):14419-27 PMID: 7142220
  15. Formation of stable preinitiation complexes between eukaryotic class B transcription factors and promoter sequences.
    Nature. 1983 Feb 24;301(5902):680-6 PMID: 6828151
  16. Mechanism of RNA polymerase II--specific initiation of transcription in vitro: ATP requirement and uncapped runoff transcripts.
    Cell. 1982 Jul;29(3):877-86 PMID: 7151173
  17. Eukaryotic gene transcription with purified components.
    Methods Enzymol. 1983;101:582-98 PMID: 6888276
  18. Multiple factors required for accurate initiation of transcription by purified RNA polymerase II.
    J Biol Chem. 1980 Dec 25;255(24):11992-6 PMID: 7440580
  19. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3855-9 PMID: 6933441
  20. Energy requirement for specific transcription initiation by the human RNA polymerase II system.
    J Biol Chem. 1984 Apr 25;259(8):5321-6 PMID: 6715344
  21. Interactions between RNA polymerase II, factors, and template leading to accurate transcription.
    J Biol Chem. 1984 Feb 25;259(4):2509-16 PMID: 6698978
  22. Purification using polyethylenimine precipitation and low molecular weight subunit analyses of calf thymus and wheat germ DNA-dependent RNA polymerase II.
    Biochemistry. 1977 May 31;16(11):2334-43 PMID: 558798
  23. Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex.
    Mol Cell Biol. 1990 Dec;10(12):6335-47 PMID: 2247058
  24. Cloning of the Schizosaccharomyces pombe TFIID gene reveals a strong conservation of functional domains present in Saccharomyces cerevisiae TFIID.
    Genes Dev. 1990 Jul;4(7):1141-8 PMID: 2210373
  25. Physical analysis of transcription preinitiation complex assembly on a class II gene promoter.
    Science. 1988 Sep 9;241(4871):1335-8 PMID: 3413495
  26. Transcription initiation by RNA polymerase II in vitro. Properties of preinitiation, initiation, and elongation complexes.
    J Biol Chem. 1987 Jan 5;262(1):298-304 PMID: 2432061
  27. Isolation of the gene encoding the yeast TATA binding protein TFIID: a gene identical to the SPT15 suppressor of Ty element insertions.
    Cell. 1989 Sep 22;58(6):1173-81 PMID: 2550146
  28. Footprinting DNA-protein complexes in situ following gel retardation assays using 1,10-phenanthroline-copper ion: Escherichia coli RNA polymerase-lac promoter complexes.
    Biochemistry. 1987 Nov 17;26(23):7234-8 PMID: 3322397
  29. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8 PMID: 3156376
  30. Mechanism and control of transcription initiation in prokaryotes.
    Annu Rev Biochem. 1985;54:171-204 PMID: 3896120
  31. Nuclease activity of 1,10-phenanthroline-copper ion. Conformational analysis and footprinting of the lac operon.
    Biochemistry. 1985 Dec 31;24(27):8050-6 PMID: 4092055
  32. Factors involved in specific transcription by mammalian RNA polymerase II. Purification and functional analysis of initiation factors IIB and IIE.
    J Biol Chem. 1987 Mar 5;262(7):3310-21 PMID: 3029109
  33. Correlation between the conformation of Escherichia coli -10 hexamer sequences and promoter strength: use of orthophenanthroline cuprous complex as a structural index.
    EMBO J. 1988 Jun;7(6):1871-9 PMID: 3049079
  34. A yeast activity can substitute for the HeLa cell TATA box factor.
    Nature. 1988 Jul 7;334(6177):77-80 PMID: 3290688
  35. Function of a yeast TATA element-binding protein in a mammalian transcription system.
    Nature. 1988 Jul 7;334(6177):37-42 PMID: 3290687
  36. Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II.
    Nature. 1989 Oct 5;341(6241):410-4 PMID: 2477704
  37. ATP activates transcription initiation from promoters by RNA polymerase II in a reversible step prior to RNA synthesis.
    J Biol Chem. 1988 Feb 25;263(6):2962-8 PMID: 2449431
  38. Factors involved in specific transcription by mammalian RNA polymerase II. Factors IIE and IIF independently interact with RNA polymerase II.
    J Biol Chem. 1989 May 25;264(15):8913-21 PMID: 2566609
  39. Stability of transcription complexes on class II genes.
    Mol Cell Biol. 1989 Jan;9(1):342-4 PMID: 2494433
  40. Cloning of the gene encoding the yeast protein BTF1Y, which can substitute for the human TATA box-binding factor.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):9803-7 PMID: 2690073
  41. Identification of a yeast protein homologous in function to the mammalian general transcription factor, TFIIA.
    EMBO J. 1989 Nov;8(11):3379-82 PMID: 2684641
  42. SPT15, the gene encoding the yeast TATA binding factor TFIID, is required for normal transcription initiation in vivo.
    Cell. 1989 Sep 22;58(6):1183-91 PMID: 2673545
  43. Dynamic interaction between a Drosophila transcription factor and RNA polymerase II.
    Mol Cell Biol. 1989 Apr;9(4):1465-75 PMID: 2725511
  44. Five intermediate complexes in transcription initiation by RNA polymerase II.
    Cell. 1989 Feb 24;56(4):549-61 PMID: 2917366
  45. Factors involved in specific transcription by mammalian RNA polymerase II: purification, genetic specificity, and TATA box-promoter interactions of TFIID.
    Mol Cell Biol. 1988 Oct;8(10):4028-40 PMID: 3185540
  46. Factors involved in specific transcription by mammalian RNA polymerase II. RNA polymerase II-associating protein 30 is an essential component of transcription factor IIF.
    J Biol Chem. 1988 Aug 5;263(22):10812-6 PMID: 3392044
  47. Is actin a transcription initiation factor for RNA polymerase B?
    EMBO J. 1984 Oct;3(10):2363-71 PMID: 6499833
  48. Variations in template protection by the RNA polymerase II transcription complex during the initiation process.
    Mol Cell Biol. 1987 Oct;7(10):3371-9 PMID: 3683385
  49. Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II.
    Cell. 1987 Nov 20;51(4):613-22 PMID: 3677170
  50. Fractionation of transcription factors for RNA polymerase II from Drosophila Kc cell nuclear extracts.
    J Biol Chem. 1987 Mar 5;262(7):3244-55 PMID: 3818640
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.17.7509
Published
1991-09-01
Pages
7509-13
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52330
Subset
IM
Grants
NCI NIH HHS · P01-CA42063 · United States
NCI NIH HHS · P30-CA14051 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.17.7509 PMC Full Text View journal details
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