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PMID: 18818313 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The steady-state level of Mg-protoporphyrin IX is not a determinant of plastid-to-nucleus signaling in Arabidopsis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 105 ·No. 39 ·2008-09-30 ·Pages 15184-9

Mochizuki N, Tanaka R, Tanaka A, Masuda T, Nagatani A

Abstract

The plastid plays a vital role in various cellular activities within plant cells including photosynthesis and other metabolic pathways. It is believed that the functional status of the plastid is somehow monitored by the nucleus to optimize the expression of genes encoding plastid proteins. The currently dominant model for plastid-derived signaling ("plastid signaling") proposes that Mg-protoporphyrin IX (MgProto) is a negative signal that represses the expression of a wide range of nuclear genes encoding plastid-localized proteins when plastid development is inhibited. In this study, we have re-evaluated this hypothesis by quantifying the steady-state levels of MgProto (as well as its neighboring intermediates protoporphyrin IX and Mg-Proto monomethyl ester [MgProtoMe]) in Arabidopsis plants with altered plastid signaling responses as monitored by expression of the Lhcb1, RBCS, HEMA1, BAM3 and CA1 genes. In addition, we have examined the correlation between gene expression and MgProto (MgProtoMe) in a range of mutants and conditions in which the steady-state levels of MgProto (MgProtoMe) have been modified. Overall we found that there was no correlation between the steady-state levels of MgProto (MgProtoMe) and Lhcb1 expression or with any of the other genes tested. Taking these results together, we propose that the current model on plastid signaling must be revised.

MeSH Terms
Arabidopsis/drug effects,genetics,metabolism,ultrastructure Arabidopsis Proteins/genetics Cell Nucleus/metabolism Gene Expression Regulation, Plant/drug effects Herbicides/pharmacology Mutation Plastids/drug effects,genetics,metabolism Protoporphyrins/metabolism Pyridazines/pharmacology Signal Transduction
Chemicals
Arabidopsis Proteins Herbicides Protoporphyrins Pyridazines protoporphyrin IX monomethyl ester norflurazone
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mochizuki Nobuyoshi
Department of Botany, Graduate School of Science, Kyoto University, Kitashirakawa, Kyoto 606-8502, Japan. mochizuki@physiol.bot.kyoto-u.ac.jp
Tanaka Ryouichi
Tanaka Ayumi
Masuda Tatsuru
Nagatani Akira
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.0803245105
Email
mochizuki@physiol.bot.kyoto-u.ac.jp
Published
2008-09-30
Epub
2008-00-25
Pages
15184-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2567512
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0803245105 PMC Full Text View journal details
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