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PMID: 18817738 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Testing odor response stereotypy in the Drosophila mushroom body.

Neuron ·Vol. 59 ·No. 6 ·2008-09-25 ·Pages 1009-23

Murthy M, Fiete I, Laurent G

Abstract

The mushroom body is an insect brain structure required for olfactory learning. Its principal neurons, the Kenyon cells (KCs), form a large cell population. The neuronal populations from which their olfactory input derives (olfactory sensory and projection neurons) can be identified individually by genetic, anatomical, and physiological criteria. We ask whether KCs are similarly identifiable individually, using genetic markers and whole-cell patch-clamp in vivo. We find that across-animal responses are as diverse within the genetically labeled subset as across all KCs in a larger sample. These results combined with those from a simple model, using projection neuron odor responses as inputs, suggest that the precise circuit specification seen at earlier stages of odor processing is likely absent among the mushroom body KCs.

MeSH Terms
Animals Animals, Genetically Modified Association Learning/physiology Behavior, Animal/physiology Drosophila/physiology Evoked Potentials/physiology Genetic Markers/physiology Mushroom Bodies/cytology,physiology Neurons, Afferent/physiology Neurons, Efferent/physiology Olfactory Pathways/cytology,physiology Patch-Clamp Techniques Smell/physiology Stereotyped Behavior/physiology Synapses/physiology Synaptic Transmission/physiology
Chemicals
Genetic Markers
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Murthy Mala
Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
Fiete Ila
Laurent Gilles
References (67)
67 references, click to expand
  1. Spatial representation of the glomerular map in the Drosophila protocerebrum.
    Cell. 2002 Apr 19;109(2):229-41 PMID: 12007409
  2. Representation of the glomerular olfactory map in the Drosophila brain.
    Cell. 2002 Apr 19;109(2):243-55 PMID: 12007410
  3. Oscillations and sparsening of odor representations in the mushroom body.
    Science. 2002 Jul 19;297(5580):359-65 PMID: 12130775
  4. New fluorescent protein reporters for use with the Drosophila Gal4 expression system and for vital detection of balancer chromosomes.
    Genesis. 2002 Sep-Oct;34(1-2):135-8 PMID: 12324968
  5. Sensory processing in the Drosophila antennal lobe increases reliability and separability of ensemble odor representations.
    Nat Neurosci. 2007 Nov;10(11):1474-82 PMID: 17922008
  6. Olfactory representations by Drosophila mushroom body neurons.
    J Neurophysiol. 2008 Feb;99(2):734-46 PMID: 18094099
  7. Visualizing glutamatergic cell bodies and synapses in Drosophila larval and adult CNS.
    J Comp Neurol. 2008 May 1;508(1):131-52 PMID: 18302156
  8. Integration of chemosensory pathways in the Drosophila second-order olfactory centers.
    Curr Biol. 2004 Mar 23;14(6):449-57 PMID: 15043809
  9. The molecular basis of odor coding in the Drosophila antenna.
    Cell. 2004 Jun 25;117(7):965-79 PMID: 15210116
  10. Molecular characterization of the singed wings locus of Drosophila melanogaster.
    BMC Genet. 2004 Jun 9;5:15 PMID: 15189568
  11. Stereotyped odor-evoked activity in the mushroom body of Drosophila revealed by green fluorescent protein-based Ca2+ imaging.
    J Neurosci. 2004 Jul 21;24(29):6507-14 PMID: 15269261
  12. Extinction antagonizes olfactory memory at the subcellular level.
    Neuron. 2002 Aug 29;35(5):951-60 PMID: 12372288
  13. Transmission of olfactory information between three populations of neurons in the antennal lobe of the fly.
    Neuron. 2002 Oct 24;36(3):463-74 PMID: 12408848
  14. Two-photon calcium imaging reveals an odor-evoked map of activity in the fly brain.
    Cell. 2003 Jan 24;112(2):271-82 PMID: 12553914
  15. Integrating the molecular and cellular basis of odor coding in the Drosophila antenna.
    Neuron. 2003 Mar 6;37(5):827-41 PMID: 12628173
  16. Development of the Drosophila mushroom bodies: elaboration, remodeling and spatial organization of dendrites in the calyx.
    Development. 2003 Jun;130(12):2603-10 PMID: 12736205
  17. The mushroom bodies of Drosophila melanogaster: an immunocytological and golgi study of Kenyon cell organization in the calyces and lobes.
    Microsc Res Tech. 2003 Oct 1;62(2):151-69 PMID: 12966500
  18. Intensity versus identity coding in an olfactory system.
    Neuron. 2003 Sep 11;39(6):991-1004 PMID: 12971898
  19. Spatiotemporal rescue of memory dysfunction in Drosophila.
    Science. 2003 Dec 5;302(5651):1765-8 PMID: 14657498
  20. Aging specifically impairs amnesiac-dependent memory in Drosophila.
    Neuron. 2003 Dec 4;40(5):1003-11 PMID: 14659098
  21. Developmental origin of wiring specificity in the olfactory system of Drosophila.
    Development. 2004 Jan;131(1):117-30 PMID: 14645123
  22. Transformation of olfactory representations in the Drosophila antennal lobe.
    Science. 2004 Jan 16;303(5656):366-70 PMID: 14684826
  23. Excitatory local circuits and their implications for olfactory processing in the fly antennal lobe.
    Cell. 2007 Feb 9;128(3):601-12 PMID: 17289577
  24. Comprehensive maps of Drosophila higher olfactory centers: spatially segregated fruit and pheromone representation.
    Cell. 2007 Mar 23;128(6):1187-203 PMID: 17382886
  25. A map of olfactory representation in the Drosophila mushroom body.
    Cell. 2007 Mar 23;128(6):1205-17 PMID: 17382887
  26. Excitatory interactions between olfactory processing channels in the Drosophila antennal lobe.
    Neuron. 2007 Apr 5;54(1):89-103 PMID: 17408580
  27. Molecular architecture of smell and taste in Drosophila.
    Annu Rev Neurosci. 2007;30:505-33 PMID: 17506643
  28. The odor coding system of Drosophila.
    Trends Genet. 2004 Sep;20(9):453-9 PMID: 15313555
  29. Conditioned behavior in Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):708-12 PMID: 4207071
  30. Neurobiology of learning and memory: the honeybee as a model system.
    Naturwissenschaften. 1983 Oct;70(10):504-11 PMID: 6656885
  31. Fiber number in the mushroom bodies of adult Drosophila melanogaster depends on age, sex and experience.
    J Neurogenet. 1984 Apr;1(2):113-26 PMID: 6085635
  32. Drosophila mushroom body mutants are deficient in olfactory learning.
    J Neurogenet. 1985 Feb;2(1):1-30 PMID: 4020527
  33. The cyclic AMP phosphodiesterase encoded by the Drosophila dunce gene is concentrated in the mushroom body neuropil.
    Neuron. 1991 Mar;6(3):455-67 PMID: 1848082
  34. Preferential expression of the Drosophila rutabaga gene in mushroom bodies, neural centers for learning in insects.
    Neuron. 1992 Oct;9(4):619-27 PMID: 1382471
  35. Mushroom bodies and Drosophila learning.
    Neuron. 1993 Jul;11(1):1-14 PMID: 8338661
  36. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
    Development. 1993 Jun;118(2):401-15 PMID: 8223268
  37. Topographic organization of sensory projections to the olfactory bulb.
    Cell. 1994 Dec 16;79(6):981-91 PMID: 8001145
  38. Information coding in the olfactory system: evidence for a stereotyped and highly organized epitope map in the olfactory bulb.
    Cell. 1994 Dec 30;79(7):1245-55 PMID: 7528109
  39. Structural plasticity in the Drosophila brain.
    J Neurosci. 1995 Mar;15(3 Pt 1):1951-60 PMID: 7891144
  40. Visualizing an olfactory sensory map.
    Cell. 1996 Nov 15;87(4):675-86 PMID: 8929536
  41. The Drosophila mushroom body is a quadruple structure of clonal units each of which contains a virtually identical set of neurones and glial cells.
    Development. 1997 Feb;124(4):761-71 PMID: 9043058
  42. Three-dimensional reconstruction of the antennal lobe in Drosophila melanogaster.
    J Comp Neurol. 1999 Mar 22;405(4):543-52 PMID: 10098944
  43. Optical imaging of odorant representations in the mammalian olfactory bulb.
    Neuron. 1999 Jul;23(3):499-511 PMID: 10433262
  44. Tripartite mushroom body architecture revealed by antigenic markers.
    Learn Mem. 1998 May-Jun;5(1-2):38-51 PMID: 10454371
  45. Development of the Drosophila mushroom bodies: sequential generation of three distinct types of neurons from a neuroblast.
    Development. 1999 Sep;126(18):4065-76 PMID: 10457015
  46. An engram found? Evaluating the evidence from fruit flies.
    Curr Opin Neurobiol. 2004 Dec;14(6):737-44 PMID: 15582377
  47. Developmentally programmed remodeling of the Drosophila olfactory circuit.
    Development. 2005 Feb;132(4):725-37 PMID: 15659487
  48. Olfactory memory formation in Drosophila: from molecular to systems neuroscience.
    Annu Rev Neurosci. 2005;28:275-302 PMID: 16022597
  49. Deconstructing memory in Drosophila.
    Curr Biol. 2005 Sep 6;15(17):R700-13 PMID: 16139203
  50. Molecular, anatomical, and functional organization of the Drosophila olfactory system.
    Curr Biol. 2005 Sep 6;15(17):1535-47 PMID: 16139208
  51. Genetic and functional subdivision of the Drosophila antennal lobe.
    Curr Biol. 2005 Sep 6;15(17):1548-53 PMID: 16139209
  52. Stereotypic and random patterns of connectivity in the larval mushroom body calyx of Drosophila.
    Proc Natl Acad Sci U S A. 2005 Dec 27;102(52):19027-32 PMID: 16357192
  53. Cholinergic synaptic transmission in adult Drosophila Kenyon cells in situ.
    J Neurosci. 2006 Jan 4;26(1):265-72 PMID: 16399696
  54. Development of wiring specificity in the olfactory system.
    Curr Opin Neurobiol. 2006 Feb;16(1):67-73 PMID: 16377177
  55. Wiring stability of the adult Drosophila olfactory circuit after lesion.
    J Neurosci. 2006 Mar 29;26(13):3367-76 PMID: 16571743
  56. Sequential use of mushroom body neuron subsets during drosophila odor memory processing.
    Neuron. 2007 Jan 4;53(1):103-15 PMID: 17196534
  57. Short-term memory in olfactory network dynamics.
    Nature. 1999 Dec 9;402(6762):664-8 PMID: 10604472
  58. Localization of a short-term memory in Drosophila.
    Science. 2000 Apr 28;288(5466):672-5 PMID: 10784450
  59. Odor maps in the mammalian olfactory bulb: domain organization and odorant structural features.
    Nat Neurosci. 2000 Oct;3(10):1035-43 PMID: 11017177
  60. Tuning and topography in an odor map on the rat olfactory bulb.
    J Neurosci. 2001 Feb 15;21(4):1351-60 PMID: 11160406
  61. Symmetry, stereotypy, and topography of odorant representations in mouse olfactory bulbs.
    J Neurosci. 2001 Mar 15;21(6):2113-22 PMID: 11245695
  62. Disruption of neurotransmission in Drosophila mushroom body blocks retrieval but not acquisition of memory.
    Nature. 2001 May 24;411(6836):476-80 PMID: 11373680
  63. Odor coding in the Drosophila antenna.
    Neuron. 2001 May;30(2):537-52 PMID: 11395013
  64. The role of Drosophila mushroom body signaling in olfactory memory.
    Science. 2001 Aug 17;293(5533):1330-3 PMID: 11397912
  65. Target neuron prespecification in the olfactory map of Drosophila.
    Nature. 2001 Nov 8;414(6860):204-8 PMID: 11719930
  66. Embryonic and larval development of the Drosophila mushroom bodies: concentric layer subdivisions and the role of fasciclin II.
    Development. 2002 Jan;129(2):409-19 PMID: 11807033
  67. Synaptic organization of the mushroom body calyx in Drosophila melanogaster.
    J Comp Neurol. 2002 Apr 8;445(3):211-26 PMID: 11920702
Article Info
Journal
Neuron
Abbr.
Neuron
ISSN
1097-4199
DOI
10.1016/j.neuron.2008.07.040
Published
2008-09-25
Pages
1009-23
Language
English
Region
United States
NLM ID
8809320
PMCID
PMC2654402
Subset
IM
Grants
NIDCD NIH HHS · R01 DC007652-01A1 · United States
Corrections
CommentIn
PMID: 18817725
External Links
PubMed source DOI: 10.1016/j.neuron.2008.07.040 PMC Full Text View journal details
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