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PMID: 188137 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Defined dimensional changes in enzyme cofactors: fluorescent "stretched-out" analogs of adenine nucleotides.

Science (New York, N.Y.) ·Vol. 195 ·No. 4275 ·1977-01-21 ·Pages 296-8

Scopes DI, Barrio JR, Leonard NJ

Abstract

A concept is presented for testing the dimensional restrictions of enzyme-active sites by stretching the substrate or cofactor by known magnitude. These restrictions of enzyme-active sites specific for purine cofactors were tested by the synthesis and evaluation of lin-benzoadenosine 5'-triphosphate, 5'-diphosphate, and 3',5'-monophosphate with respect to enzyme binding and activity. These "stretchedout" (by 2.4 angstroms) versions of the adenine ribonucleotides bind strongly, slow the enzymatic rates, and have useful fluorescence properties.

MeSH Terms

Adenine Nucleotides/metabolism Adenosine Diphosphate/analogs & derivatives Adenosine Triphosphate/analogs & derivatives Animals Binding Sites Catalysis Chemical Phenomena Chemistry Coenzymes/metabolism Hexokinase/metabolism In Vitro Techniques Phosphofructokinase-1/metabolism Phosphoglycerate Kinase/metabolism Phosphotransferases/metabolism Polyribonucleotide Nucleotidyltransferase/metabolism Protein Binding Pyruvate Kinase/metabolism Rabbits Saccharomyces cerevisiae Spectrometry, Fluorescence Structure-Activity Relationship

Chemicals

Adenine Nucleotides Coenzymes Adenosine Diphosphate Adenosine Triphosphate Phosphotransferases Hexokinase Phosphofructokinase-1 Pyruvate Kinase Phosphoglycerate Kinase Polyribonucleotide Nucleotidyltransferase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Scopes D I

Barrio J R

Leonard N J

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.188137

Published

1977-01-21

Pages

296-8

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.188137 View journal details

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Defined dimensional changes in enzyme cofactors: fluorescent "stretched-out" analogs of adenine nucleotides.

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